摘要
目的:优化黄芩愈伤组织培养条件。方法:采用均匀设计法,以黄芩甙含量为主要考察指标,对黄芩愈伤组织生长的MS培养基成分进行多因素多水平考察。结果:最佳培养基为MS附加0.25mg/L NAA、0.5mg/L 6-BA、17g/L葡萄糖和35g/L蔗糖。最佳培养基黄芩甙含量为27.44mg/gDW。结论:用均匀设计法优化愈伤组织培养基省时、方便,且最佳培养基黄芩甙含量与预测值相近,且明显高于均匀设计实验方案中的黄芩甙含量。经SAS软件分析得出的优化方程拟合度很好。
Objective： To optimize the medium for Scutellaria Baicalensis Georgi callus. Methods： The components of MS medium for growth of callus were investigated from poly- factors and poly - levels by uniform design. Baicalin content was chosen as the main experiment index. Restilts： The optimum medium was MS added 0.25mg/L NAA, 0.5mg/L 6 - BA, 17g/L glucose and 35g/L sucrose. The optimum baiealin content was 27.44 mg/gDW. Conclusion： Uniform design is a saving, convenient method for the optimization of medium for callus. The optimum medium had the similar baicalin content to the expected and more than that of the arrangements in uniform design. The optimization equation obtained by the SAS was fit for the experiment.
出处
《生物技术》
CAS
CSCD
2006年第6期62-64,共3页
Biotechnology
基金
黑龙江省普通高等学校骨干教师创新能力计划项目资助(No.1054G066)
关键词
黄芩
愈伤组织
均匀设计
黄芩甙
ScuteUaria Baicalensis Georgi
callus
uniform design
baicalin
