摘要
目的:艾溃灵合剂为临床用于口腔溃疡疗效确切的中药制剂,采用动物实验,观察其抗炎效应。方法:实验于2005-02/10在河南中医学院动物实验中心完成。艾溃灵合剂主要由黄连、连翘、黄芪、生地、车前草、甘草等药组成。①将50只昆明小鼠按随机数字表分为5组:大、中、小剂量艾溃灵合剂组(20,10,5g原生药/mL),口炎清颗粒组(3.3g成品)及同体积的生理盐水组,每组10只。灌服1次/d,连续给药3d,于最后1次给药后1h,每鼠右耳涂二甲苯,脱颈椎处死小鼠,剪下双耳,用打孔器打下双耳耳片,迅速用电子天平称质量,并计算肿胀度。肿胀度=右耳质量-左耳质量。②将40只wistar大鼠按随机数字表分为5组:大、中、小剂量的艾溃灵合剂组(20,10,5g原生药/mL),口炎清颗粒组(3.3g成品)及同体积的生理盐水组,每组8只。灌服给药1次/d,连续给药3d,于最后1次给药后30min,于每鼠左、右后足跖皮下注射新配制的10%新鲜鸡蛋清液,分别于给蛋清后30min、60min、120min、240min、360min在足跖测定仪再次测大鼠左右后足跖体积,并计算足跖肿胀率。结果:90只大小鼠均进入结果分析。①与生理盐水组相比,大、中、小剂量艾溃灵合剂组和口炎清颗粒组均可显著抑制小鼠耳壳二甲苯致肿,使耳壳肿胀度显著降低[(7.0±1.6),(2.3±1.5),(3.5±1.5),(4.7±1.4),(3.6±1.5)mg,P<0.01]。②与生理盐水组比,大、小剂量艾溃灵合剂组在给药后30～360min均可显著抑制大鼠蛋清性足跖肿胀[生理盐水组:0.73±0.12,0.67±0.12,0.54±0.12,0.40±0.14,0.23±0.12;大剂量艾溃灵合剂组:0.56±0.11,0.49±0.09,0.36±0.10,0.27±0.13,0.07±0.07;小剂量艾溃灵合剂组:0.63±0.13,0.54±0.14,0.44±0.13,0.27±0.15,0.12±0.13,P<0.01,P<0.05),中剂量艾溃灵合剂组在给药后30min、120min、240min可明显抑制大鼠蛋清性足跖肿胀,在给药后60min和360min可显著抑制大鼠蛋清性足跖肿胀[生理盐水组:0.73±0.12,0.54±0.12,0.40±0.14,0.67±0.12,0.23±0.12;中剂量艾溃灵合剂组:0.59±0.16,0.43±0.16,0.27±0.13,0.52±0.13,0.11±0.11,P<0.05,P<0.01),口炎清颗粒组在给药后30～360min均可明显抑制大鼠蛋清性足跖肿胀[生理盐水组:0.73±0.12,0.67±0.12,0.54±0.12,0.40±0.14,0.23±0.12;口炎清颗粒组:0.64±0.07,0.56±0.11,0.43±0.10,0.30±0.08,0.12±0.10,P<0.05]。结论:艾溃灵合剂可显著抑制小鼠耳壳二甲苯致肿,显著抑制大鼠蛋清性足跖肿胀,具有明显的抗炎作用。
AIM： Aikuiling liquid is a preparation of Chinese traditional medicine that has a good curative effect on buccal ulceration. To observe the anti-inflammatory effects of the Aikuiling liquid in animal experiment. METHODS： The experiment was accomplished in the Animal Experiment Center of the Henan College of Traditional Chinese Medicine From February to October 2005. Aikuiling was composed of coptidis rhizoma, forsythia suspense, astragalus mongholicus, rehmannia dride rhizome, plantain, licorice root and so on. ①Dividing the 50 Kunming mice into 5 groups： the large, middle, small dose （20,10,5 g crude drug/mL） of Aikuiling groups, the Kouyanqing granule group （3.3 g finished product） and saline group with 10 in each group, once a day for 3 days successively. One hour after giving the medicine for the last time, embrocated the mice＇s right ear with dimethylbenzene, executed the animals by casting off cervical vertebra, cut off the two ears, used the stiletto to get two pieces of the ears, weighed them quickly, and then counted the degree of tumefaction. Degree of tumefaction was equal to that mass of right ear reduced mass of left ear.②Dividing the 40 Wistar rats into 5 groups： the large, middle, small dose of Aikuiling groups （20,10,5 g crude drug/mL）, the Kouyanqing granule group （3.3 g finished product） and saline group with 8 in each group, once a day, for 3 days successively. 30 minutes after giving the medicine for the last time, the 10% liquid of egg white was hypodermically injected into left and right back voix pedis of rats. At minutes 30, 60, 120, 240 and 360 the bulk of voix pedis was measured and the degree of tumefaction was calculated. Degree of tumefaction was equal to （the bulk of voix pedis at different times after administration-the bulk of normal voix pedis at the same side）/the bulk of normal voix pedis at the same side. RESULTS： A total of 90 mice and rats were involved in the result analysis.①Compared with the saline group, the large, middle, small dose of Aikuiling groups and the Kouyanqing granule group could remarkably reduce the tumefaction of ear of mice caused by dimethylbenzene [（7.0±1.6）, （2.3±1.5）, （3.5-±1.5）, （4.7±1.4）,（3.6±1.5） mg,P 〈 0.01]. ②Compared with the saline group, at the time of 30-360 minutes, the large and small dose of A ikuiling groups could remarkably i＇educe the tumefaction of rats＇ voix pedis caused by egg white [saline group （0.73±0.12）,（0.67±0.12）, （0.54±0.12）, （0.40 ±0.14）, （0.23±0.12）;large dose of Aikuiling group （0.56±0.11）, （0.49±0.09）, （0.36±0.10）, （0.27±0.13）, （0.07±0.07） ;small dose of Aikuiling group （0.63±0.13）, （0.54±0.14）, （0.44±0.13）, （0.27±0.15）, （0.12±0.13）, P 〈 0.01,P 〈 0.05）. The middle dose of Aikuiling group could＇reduce the tumefaction of rats＇ voix pedis caused by egg white at the time of 30, 120 and 240 minutes, and could obviously reduce the tumefaction of rats＇ voix pedis caused by egg white at the time of 60 and 360 minutes [saline group： 0.73±0.12,0.54±0.12,0.40±0.14,0.67±0.12,0.23±0.12 ; middle dose of Aikuiling group ：0.59 ±0.16,0.43 ±0.16,0.27 ±0.13,0.52 ±0.13,0.11±0.11,P 〈 0.05,P 〈 0.01）. The Kouyanqing granule group could obviously reduce the tumefaetion of rats＇voix pedis caused by egg white at the time of 30-360 minutes [saline group：0.73±0.12,0.67±0.12, 0.54 ±0.12,0.40 ±0.14,0.23 ±0.12;Kouyanqing granule group：0.64 ±0.07, 0.56±0.11,0.43±0.10,0.30±0.08,0.12±0.10,P 〈 0.05）. CONCLUSION： Aikuiling liquid can remarkably reduce the tumefaction of miee＇s earlap caused by dimethylbenzene and the tumefaetion of rats＇ voix pedis caused by egg white. AiKuiLing liquid has obviously anti-inflammatory effects.
出处
《中国临床康复》
CSCD
北大核心
2006年第47期101-103,共3页
Chinese Journal of Clinical Rehabilitation
基金
国家"十五"科技攻关艾滋病专项(2004BA719A09-0204)
国家"十五"省部联动项目(2004BA719A13-06)~~
