摘要
目的：观察雷米普利时急性肺损伤(ALI)时肺组织核因子-κB(NF-κB)活性的影响。方法：SD大鼠随机分为正常对照组(NS组)、内毒素损伤组(LPS组)和雷米普利预防组(RAM组),用免疫组织化学法检测肺组织NF-κB的表达,并进行肺系数测定以及肺组织炎症细胞计数；光镜下观察大鼠肺组织的病理形态学变化。结果：LPS组肺组织NF-κB表达比NS组增强(P＜0．05),肺系数和炎症细胞数比NS组增加(P＜0．05),肺组织损伤改变严重；RAM组NF-κB表达比LPS组降低(P＜0．05),肺系数和炎症细胞数较LPS组减少(P＜0．05),肺组织损伤程度也减轻。结论：NF-κB活化在LPS诱导的AL1中起重要作用,雷米普利可降低NF-κB的活性,减轻LPS导致的ALI。
Objective To observe the effect of Ramipril on the expression of NF-κB in acute lung injury （ALI）, Methods： SD Rats were divided randomly into three groups; control group, LPS group and RAM group. The expression of NF-κB in the lung tissue was measured by immunohistoehemistry, the lung coefficient was examined and took count of inflammatory cell in the lung tissue. The pathomorphologic changes of lung tissue were examined. Results： The expression of NF-κB in the LPS group was significantly higher than that in the control group （P〈0. 05）, the lung coefficient and the inflammatory cell in the lung tissue were higher than in the control group （P〈0. 05）, and the pathomorphology in lung tissue showed sever injuries. Expression of NF-κB was significantly lower in the RAM group compared with that in the LPS group （P〈0. 05）, the lung coefficient and the inflammatory cell in the lung tissue were decreased than those in the LPS group （P〈0. 05）, and the lung injury was less,too. Conclusion： NF-κB plays a critical role in LPS-induced ALI. Ramipril may lower the expression of NF-κB and relieve LPS-induced ALI.
出处
《内科急危重症杂志》
2006年第6期273-274,296,F0003,共4页
Journal of Critical Care In Internal Medicine
