摘要
目的探讨非离子表面活性剂聚氧乙烯醚聚乙二醇300曲拉通X100对肺癌耐药细胞A549/DDP(顺铂)多药耐药性的逆转及其机制。方法用MTT法进行体外耐药逆转实验,荧光分光光度计测定细胞内ADM药物浓度。结果合用非离子型表面活性剂前后,A549／DDP对化疗药物阿霉素、顺铂、丝裂霉素、5-FU、依托泊甙和长春新碱的敏感性均有显著性差异(各组都为P<0．01);三种非离子型表面活性剂都能显著增加耐药细胞内ADM浓度,实验组与对照组比较差异有显著性(P<0．01)。结论三种非离子表面活性剂具有逆转A549/DDP耐药性的作用,逆转机制可能是改变细胞膜的流动性,抑制多药耐药相关膜蛋白的功能。
Objective To discuss whether the non-ionic surfactant （Cremophor EL, PEG300, TritonX-100）can reverse the MDR of lung cancer cell strian A549/DDP or not and its mechanism. Methods The reverse of drug resistance was studied by MTT fluorospectorphotometer was used to detect the concentration of ADM（阿毒素） in cells. Results MTT cytotoxic assay revealed that the sensitivities of A549/DDP cells to chemotherapy drugs were different according to the presence or absence of non-ionic surfactant （ P 〈 0.01 ） non- ionic surfactant significantly increased the concentration of ADM in drug - resistant cell, the result has significant difference（P 〈 0.01）. Conclusion The drug resistance of A549/ DDP cells can be reversed by three non-ionic surfactant, its mechanism is that non-ionic surfactant can change cell membrane fluidity and inhibit some function of membrane proteins to cause MDR.
出处
《同济大学学报（医学版）》
CAS
2006年第6期16-18,共3页
Journal of Tongji University(Medical Science)
基金
上海市科委课题基金资助项目(03DZ19236)
