摘要
目的研究正常角质形成细胞分泌的TGF-β1对正常成纤维细胞生物学行为的影响。方法采用免疫组化方法检测角质形成细胞分泌TGF-β1,以不同浓度TGF-β1抗体阻断角质形成细胞条件培养液中的TGF-β1,观察阻断前后角质形成细胞条件培养液对正常成纤维细胞增殖,胶原分泌的影响。结果细胞玻片可见大量染色阳性角质形成细胞,经TGF-β1抗体阻断后的角质形成细胞条件培养液,对成纤维细胞的促增殖作用明显减弱,胶原分泌减少。结论正常角质形成细胞分泌大量TGF-β1,可促进成纤维细胞增殖,促进胶原分泌。
Objective To observe the effects of TGF - β1 derived from keratinocytes on proliferation and collagen secretion of fibroblasts. Methods The immunohistochemisty method was employed to confirm whether the keratinocytes could secrete TGF - β1. Conditioned medium of keratinocytes with different concentration TGF- β1 antibody was added to the fibroblasts. Cell counting kit - 8 and radiohistology method were employed to measure the cell proliferation and collagen secretion. Results Keratinocytes did secret TGF-β1. there was a significant difference in fibroblasts proliferation between keratinocytes conditioned medium and medium with TGF-β1 antibody, the same as the collagen secretion. Conclusion Keratinocytes can secret TGF-β1 which can increase fibroblasts proliferlation and collagen secretion.
出处
《中国美容整形外科杂志》
CAS
2006年第6期467-469,共3页
Chinese Journal of Aesthetic and Plastic Surgery
基金
国家自然科学基金资助项目(30371469)
