摘要
目的:探讨精子早期凋亡过程中质膜磷脂酰丝氨酸(PS)外翻与线粒体膜电位丧失的相关性。方法:42例精液标本用AnnexinV/PI和Rh123/PI双染法染色后,用流式细胞术(FCM)分析。结果:AnnexinV/PI染色法中,与正常生育组比,不育组活精子和死精子百分率均存在统计学差异(P=0.001,P=0.003),凋亡精子百分率无统计学差异。Rh123/PI染色法中,与正常组相比,线粒体膜电位正常精子百分率和死精子百分率均存在统计学差异(P=0.000,P=0.001),线粒体膜电位丧失精子百分率差异无显著性(P=0.120)。两组中凋亡精子百分率与线粒体膜电位丧失精子百分率间均呈正相关。结论:精子存在质膜PS外翻和线粒体膜功能紊乱,且两者间存在相关性。
Objective： To study whether a relationship exists between sperm plasma membrane translocation of phosphatidylserine （PS） and loss of mitochondrial transmembrane patential during early apoptosis.Methods： Forty-two semen samples from the male partners of couples were classified as normal （n=10） and abnormal （n=32）. Rh123/PI and Ann exin V/PI dual fluorescent staining were then carried out in order to analyze by flow cytometry（FCM）. Results： The significant differences in the percentage of active sperm and dead sperm were detected between the subjects with semen of fertile and infertile men （P〈0.05）, no significant differences in the percentage of apoptotic sperms were detected. In Rh123/PI staining, the significant differences in the percentage of sperm with loss of mitochondrial transmembrane potential （ IIB type） and dead sperm were detected between the subjects with semen of fertile and infertile men （P〈0.05）, no significant differences in the percentage of sperm （ Ⅱ A type） were detected. There was a positive correlation in both fertile and infertile groups between the percentages of apoptotic sperm （Ⅱ A type） and ⅡB type sperm （r=0.759 and r=0.622）. Conclusion： Plasma membrane translocation of PS and loss of mitochondrial transmembrane potential can be easily detected by FCM, both of which are markers of early apoptosis. Positive correlation is seen between them.
出处
《生殖与避孕》
CAS
CSCD
北大核心
2006年第12期735-739,共5页
Reproduction and Contraception
基金
江苏省医学重点学科基金资助(批准号:苏卫科教[2001]34)
