摘要
生物体内一氧化氮和超氧阴离子(.O2-)快速反应生成的过氧亚硝酸根离子(ONOO-)是一种强细胞毒性物质,它能够硝化胰岛素的酪氨酸残基。采用UV-Vis和Native-PAGE等方法,在体外研究了不同因素对ONOO-硝化胰岛素的影响。结果表明,在生理pH值条件下,CO2/HCO3-对胰岛素的硝化有催化作用,而葡萄糖和血清白蛋白以及一些抗氧化剂却对胰岛素的硝化有抑制作用。
Peroxynitrite （ONOO^-） is a potent cytotoxic agent produced by rapid combination of NO and · O2^- , and can nitrate the tyrosine residues of insulin. The effects of some important factors on the nitration of insulin by peroxynitrite were studied with UV-Vis and Native-PAGE in vitro. Present results show that CO2/ HCO3^- catalyzes the nitration of insulin, but glucose, albumin and some antioxidants inhibit the nitration in physiological pH value.
出处
《化学与生物工程》
CAS
2006年第12期29-31,共3页
Chemistry & Bioengineering
基金
国家自然科学基金项目(20371018)
