摘要
尖吻蝮蛇毒和蝮蛇毒凝血酶样酶促凝的协同作用魏文利１，孙家钧，陈家树（中山医科大学药理教研室，广州５１００８９，中国）关键词尖吻蝮蛇；蝮蛇；蛇毒；凝血酶类；血液凝固目的：尖吻蝮蛇（ＤＡ）和蝮蛇（ＡＨ）蛇毒凝血酶样酶（ＴＬＥ）对促凝作用的协同效果．方法：...
IM: To study the synergistic effect of
thrombin like enzymes (TLE) of Dienagkistrodon acutus (DA) and
Agkistrodon halys (AH) venoms. METHODS: TLE were isolated from
venoms of DA and AH by successive column chromato￣graphy.
Effects of combination of DATLE and AHTLE and related factors on the
clotting time and clot quality were tested in vitro . RESULTS: One
coagulation unit was 2 7 μg for DATLE and 304 4 μg, for AHTLE
with reference standard of thrombin (42 2 μg). TLE induced clot
was a fibrin monomer which was fragile and did not retract.
Combination of AHTLE and DATLE shortened the clotting time and
decreased the solubility of the clot in urea 5 mol·L -1 . When
subthreshold concentration of thrombin or physiological concentration
of Ca 2+ was added, the clotting time was further shortened, the
clot was no longer soluble in urea and retracted well, and the
resistance of the clot to plasmin degradation was increased.
CONCLUSION: A synergistic effect of DATLE and AHTLE accelerated
hemocoagulation and improve clot quality.
出处
《中国药理学报》
CSCD
1996年第6期527-531,共5页
Acta Pharmacologica Sinica
关键词
尖吻蝮蛇
蝮蛇
蛇毒
凝血酶类
血液凝固
Dienagkistrodon acutus
Agkis￣trodon halys
snake venoms
thrombin
blood coagulation
