摘要
目的对北京儿童医院的头癣患者分离所得的182株犬小孢子菌进行抗真菌药敏试验条件的探索。方法对致病真菌进行取样、培养、鉴定、分离和纯化，经微量稀释法测定8种常见抗真菌药物的最小抑菌浓度，并对原药敏方案进行适当调整。结果受试菌株的接种浓度为1—3×10^4 CFU／mL。除伊曲康唑、联苯苄唑、阿莫罗芬外，使用沙氏液体培养基测定的药物最小抑菌浓度均较使用RPMI-1640液体培养基时药物浓度明显升高。孵育温度为28℃，孵育时间为5d。结论各种药物的最小抑菌浓度（minimal inhibitory concentration，MIC）之间的差异有统计学意义，M38-A方案有较好的重复性和稳定性，可作为一种较好的实验室方法被应用。
Objective Study on the broth microdilution antifungal susceptibility testing condition about 182 Microsporum canis derived from patients in Beijing Children＇ s Hospital. Methods The vitro method was used for testing filamentous fungi. We approached the broth microdilution antifungal susceptibility testing condition for Microsporum canis and tested the minimal inhibitory concentration （MIC） of 8 common antifungal drugs. Results The broth microdilution antifungal susceptibility testing condition for Microsporum canis ： inoculum size ranged from 1 × 10^4 to 3 × 10^4 CFU/mL; MICs obtained from different medium at the same incubation time for the same isolate were significantly different, MICs were consistently higher with SDB than with RPMI-1640, when itraconazole, bifonazole, amorolfine were tested, no parameter had any influence on MICs ; incubation temperature was 28 ℃, Microsporum canis produced clearly detectable growth after 5 d. Conclusion With good reproducibility and feasibility, the M38-A procedure can be used for the antifungal susceptibility test to dermaphytes in the laboratories.
出处
《首都医科大学学报》
CAS
2006年第6期750-753,共4页
Journal of Capital Medical University
