期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

几种化疗药物对肝癌耐药细胞BEL7404/ADM端粒酶活性的影响

Effects of different chemotherapeutic drugs on telomerase activity of human hepatocellular carcinoma drug-resistance BEL-7404/ADM cell line
下载PDF
导出
摘要 目的:研究顺铂(DDP)、丝裂霉素(MMC)、多柔比星(ADM)和长春新碱(VCR)4种化疗药物对人肝癌细胞株BEL-7404、耐阿霉素肝癌细胞株BEL-7404/ADM的细胞毒作用及其对端粒酶活性影响的关系。方法:采用MTT法测定常用4种(DDP、MMC、ADM和VCR)化疗药物对人肝癌细胞株BEL-7404、BEL-7404/ADM的体外抑制作用;以TRAP-PCR-ELISA法检测化疗药物作用后两种细胞端粒酶的活性。结果:DDP、MMC、ADM、VCR对BEL-7404均敏感,各种化疗药物IC50大小比较,DDP和ADM比VCR和MMC均小;而对BEL-7404/ADM这几种化疗药物敏感性均不同程度下降,其中以ADM及VCR尤其明显,耐药倍数分别为100·0倍、60·14倍。未加药前,肝癌亲本细胞BEL-7404和耐药细胞BEL-7404/ADM端粒酶活性均表达,加入化疗药物后,亲本细胞端粒酶活性下降明显,并呈现时间-剂量关系,而对于耐药细胞,只有高浓度的化疗药物才能抑制其端粒酶活性。结论:端粒酶活性的抑制与耐药细胞对药物敏感性存在一定关系,端粒酶可作为化疗敏感的潜在标志。 OBJECTIVE：To evaluate the relationship between telomerase activity and drug-sensitivity in human hepatocellular carcinoma cell lines. METHODS： The human hepatocellular carcinoma drug-sensitive cell line BEL- 7404 and drug-resistance cell line BEL-7404/ADM were treated of chemotherapeutic drugs （cisplatin DDP, adriamycin ADM,mitomycin MMC, VCR respectively）. MTT was used to measure the multiplier of drug resistance. Telomerase activity was detected by TRAP-PCR-EI.ISA. RESULTS： The drug resistance indexes of hepatocellular carcinoma cell lines BEL-7404/ADM to ADM and MMC were 100 and 60 respectively. The telomerase activity in BEL-7404 was decreasing with the increasing of DDP, ADM, VCR, MMC concentration but not so apparent in BEL-7404/ADM. Only high concentration of DDP, ADM, MMC and VCR deregulated telomerase activity of BEL-7404/ADM in a time-depending manner. CONCLUSION ： Telomerase activity is related to the drug-sensitivity in hepatocellular carcinoma drug-resistance cell. Telomerase is a potential chemosensitive marker.
作者 陈艳华 黎丹戎 韦长元 莫军扬
机构地区 广西肿瘤防治研究所
出处 《中华肿瘤防治杂志》 CAS 2006年第20期1545-1548,共4页 Chinese Journal of Cancer Prevention and Treatment
基金 广西自然科学基金(桂科攻0229040)
关键词 肝肿瘤/酶学 端粒 末端转移酶 抗肿瘤药物/药理学 比色法 liver neoplasms/enzymologic telomerase antineoplastic agents/pharmacology colorimetry
分类号 R735.7 [医药卫生—肿瘤]
  • 相关文献

参考文献13

  • 1Meyerson M.Role of telomerase in normal and cancer cells[J].J Clin Oncol,2000,18(13):2626-2634.
  • 2Helder M N,Wisman G B,Van der Zee G J.Telomerase and telomers from basic biology to cancer treatment[J].Cancer Invest,2002,20(1):82-101.
  • 3orin G B.The human telomere teminal transferase enzyme is a ribonucleoprotein that synthesizes TTAGG repeats[J].CELL,1985,43(2):405-413.
  • 4Shay J W,Bacchetti S.A survey of telomerase activity in human cancer[J].E JC,1997,33(3):787-795.
  • 5陈宏,谢兆霞,肖希斌,王光平.Survivin反义寡核苷酸逆转K562/A02细胞的耐药研究[J].中华肿瘤防治杂志,2006,13(6):423-425. 被引量：2
  • 6Kondo Y,Kondo S,Tanake Y,et al.Inhibition of telomerase increases the susceptibility of human malignant glioblastoma cells to cisplatin induced apoptosis[J].Oncogene,1998,16 (17):2243-2246.
  • 7Kunifuji,Yasumasa,Gotoh,et al.Down-regulation of telomerase activity by anticancer drugs in human ovarian cancer cells[J].AnTicancer Drugs,2002,13(6).595-598.
  • 8Nason-Burchenal K,Maerz W,Albanell J,et al.Common defects of different retinoic acid resistant promyelocytic leukemia cells are persistent telomerase activity and nuclear body disorganization[J].Differentiation,1997,61(5):321-331.
  • 9Zhang W,Piatyszek M A,Kobayashi T,et al.Telomerase activity in human acute myelogeneous leukem:inhibition-inducing agents[J].Clin Cancer Res,1996,2(5):799-803.
  • 10Terashima M,Takiyama I,Uesugi N,et al.Telomerase assay as a possible predictor of the response to anticancer chemotherapy[J].An ticancer Res,2000,20(1A):293-298.

二级参考文献18

  • 1Nakamura T M,Morin G B.Chapman K B .et al.Telomerase catalytic subunit homologs from fission yeast and human[J].Science,1997.277(33):955-959.
  • 2Engelberg H.Actions of heparin that may affect the malignant process[J].Cancer,1999.85(2):257-272.
  • 3Borsig L.Wong R.Feramisco J,et al.Heparin and cancer revisited:mechanistic connections involving platelets,P-selectin.carcinoma mucis,and tumor metastasis[J].Proc Natl Acad Sci U S A.2001,98(6):3352-3357.
  • 4Varki N M,Varki A.Heparin inhibition of selectin-Mediated interactions during the hematogenous phase of carcinoma metastasis:rationale for clincal studies in humans[J].Semin Thromb Hemost,2002,28(1):53-66.
  • 5Yamaguchi F,Morrison R S.Takahashi H,et al.Anti-telomerase therapy suppressed glioma proliferation[J].Oncol Rep,1999.6(4):773-776.
  • 6Kunifuji Y.Gotoh S,Abe T,et al.Down-regulation of telomerase activity by anticancer drugs in human ovarian cancer cells[J].Anticancer Drugs,2002,13(6):595-598.
  • 7Rowley P T,Tabler M.Telomerase inhibitors[J].Anticancer Res,2000,20(6):4419-4429.
  • 8Lei W,Mayotte J E,Levitt M L.Enhancement of chemosensitivity and programmed cell death by tyrosine kinase inhibitors correlates with EGFR in non small cell lung cancer cell[J].An ticancer Res,1999,19(A):221 -228.
  • 9Olie R A,Simoes-Wust A P,Baumann B,et al.A novel antisense oligonucleotide targeting survivin expression induces apoptosis and sensitizes lung cancer cells to chemotherapy[J].Cancer Res,2000,60(1):2805-2809.
  • 10Altieri D C.The molecular basis and potential role of survivin in cancer diagnosis and therapy[J].Trends Mol Med,2001,7(12):542-547.

共引文献6

中华肿瘤防治杂志
2006年 第20期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部