摘要
采用HPLC法测定了白花丹10批样品。色谱条件:Krosm asil C18柱(250 mm×4.6 mm,5μm),甲醇-水梯度洗脱,检测波长270 nm,流速1 mL/m in,柱温30℃。以主成分分析法除去离异样品后,建立了白花丹药材HPLC标准指纹图谱。建立白花丹的HPLC标准指纹图谱,为科学评价白花丹药材质量提供了新方法。
HPLC （high performance liquid chronmtography ） was used to analyze 10 batehes of Plumbago zeylaniea 1,. samples from Guangxi and Yunnan provinces. The ItPLC fingerprict separation was performed on a Krosmatsil C18 analytical column gradient eluted with methanol - water at a flow rate of 1mL/min. The temperature of the column was set at 30℃ and the UV detection wavelength was 270 nm. The deviant samples were removed with principal cnmponenl analysis and the standard HPLC fingerprints for P. zeylanica were set up.
出处
《西南农业大学学报（自然科学版）》
CSCD
北大核心
2006年第6期930-932,共3页
Journal of Southwest Agricultural University
基金
四川省青年基金资助项目(2007)
四川省教育厅教育教学改革资助项目(2005)
关键词
白花丹
HPLC
指纹图谱
Guangxi and Yunnan provinces
Plumbago zeylanica L.
HPLC
fingerprint
