摘要
240羽肉鸡于14日龄时随机分为常温对照组(A)、低温对照组(B)和低温呋喃苯胺酸添加组(C)。分别于14、23、30、37、44日龄从各组随机抽取10羽肉鸡采血和扑杀,测定肺血管管壁面积与管总面积之比(WA/TA)、平均中膜厚度(mMTPA)等各项指标。观察在低温环境下呋喃苯胺酸对肉鸡肺动脉高压综合征(PHS)的防治效果及其对肉鸡肺血管重构的影响。试验结果显示,与A组相比,B组PHS发病率升高,右心全心比(RV/TV)、红细胞压积(PCV)和反映血管重构指标的WA/TA和mMTPA值显著升高(P<0.05);而C组与B组相比,PHS发病率降低,RV/TV、WA/TA和mMTPA值均降低,并在44日龄差异显著(P<0.05),PCV值升高但无显著差异,体质量显著降低。提示:降低肺动脉压,抑制以肺动脉壁肥厚为特征的血管重构可能是呋喃苯胺酸有效降低低温所致PHS发病率的原因之一。
The objective of the present study was to evaluate the influence of dietary furosemide （FURO） on the incidence of pulmonary hypertension syndrome （PHS） induced by cool ambient temperature and to investigate its effect on the pulmonary vascular remodeling of broiler chickens. A total of two hundred and forty broilers were randomly allocated to three groups. The birds in Group A were fed with a grower diet and kept in normal ambient temperature from 14 to 44 days. Group B and Group C were subjected to cool ambient temperature （12-14℃） to induce PHS and were fed with a grower diet with （Group C） or without （Group B） 150 mg·kg^-1 furosemide from 30 to 44 days. PHS incidence, body weight were measured weekly for each group. Heart, lung and blood samples were taken from 10 birds per group randomly selected at 14, 23, 30, 37 and 44 days of age, for the determination of the ratio of vessel wall area to total area （WA/TA）, mean medial thickness in pulmonary arterioles （mMTPA）, etc. It was found that birds exposed to cold environment had higher PHS morbidity. However, it was reduced by dietary furosemide supplementation. The results showed that： total ventricular weight ratios （RV/TV） and packed red blood cell volume （PCV） in Group B were significantly higher than Group A, also were the value of WA/TA and mMTPA which served as describing pulmonary vessel remodeling. Birds fed 150 mg·kg^-1 furosemide had significantly lower RV/TV （P 〈 0.05） than the birds in Group B, The values of WA/TA and mMTPA were significantly reduced （P 〈 0.05）, body weight was reduced too, whereas PCV was increased. It was concluded that dietary furosemide reduced morbiciity of PHS induced by cool ambient temperature, presumably by reducing pulmonary arterial pressure. Attenuated pulmonary vascular remodeling might be involved in the underlying mechanisms too.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2006年第4期97-101,共5页
Journal of Nanjing Agricultural University
基金
国家自然科学基金项目(30371061
30571366)
江苏省"九五"农业重点攻关项目(BE964888)
关键词
肉鸡
肺动脉高压综合征
呋喃苯胺酸
血管重构
broiler
pulmonary hypertension syndrome （PHS）
furosemide
vascular remodeling
