摘要
对分离到的一株高抗Cu(200 mm ol/L CuSO4.5H2O)的P en icillium janth inellum菌株GXCR(中国微生物菌种保藏管理委员会普通微生物中心保藏号:CGM CC 1027)进行紫外诱变,获得了3株Cu抗性下降突变体,进一步测定结果表明,3个突变体对A l[A l2(SO4)3]和Zn(ZnSO4.7H2O)的抗性也显著下降;只有在含Cu的PDA上生长的菌体表面能够形成CuSO4.5H2O样的蓝色晶体。进一步的X-ray衍射的原子微量分析表明,这种蓝色晶体含Cu2+,说明该菌具有Cu的生物矿化作用。菌体表面积累的Cu2+的量与菌株的抗Cu水平有正相关性,说明该菌对Cu的抗性很可能与Cu矿化作用有关。
A Cu-resistant （200 mmol/L CuSO4 · 5H2O） Penicillium janthinellum strain,designated GXCR （ General Microbiological Culture Collection Center, Beijing, China. CGMCC No. 1027） , was mutagenized with ultraviolet （UV） radiation. Three mutants with significant decrease in Cu resistance were generated. Further assay revealed that these mutants showed significant decrease in resistance to Al [Al2 （SO4）3] and Zn （ZnSO4 · 7H2O）. It was found that only colonies that could grow on Cu- containing PDA plate produced CuSO4 · 5H2O like blue crystals. Microanalysis of metal atoms by X- ray indicated that this kind of blue crystals contained Cu^2＋ , suggesting that this fungus has the ability for Cu biomineralization. Amount of Cu^2＋ accumulated on mycelial surfaces correlated positively with levels of Cu resistance , suggesting that resistance of this fungus to Cu is likely to be related to its Cu biomineralization .
出处
《广西农业生物科学》
CAS
CSCD
2006年第4期294-299,共6页
Journal of Guangxi Agricultural and Biological Science
基金
广西区科技厅项目(桂科能0443001-20)
关键词
青霉菌
金属盐
抗性
生物矿化
Penicillium janthinellum
metal salt
resistance
biomineralization
