摘要
目的研究甲醛对Hela细胞GSNO还原酶(GSNOR)活力的影响及其与氧化胁迫的关系。方法Hela细胞暴露于不同浓度液态甲醛和100μmol/ml的H2O2,染毒1h,然后检测细胞裂解液中GSNOR酶活力。结果甲醛可依剂量-效应曲线的方式激活GSNOR,导致酶活力升高,其中,高浓度甲醛(1250μmol/ml)可导致GSNOR活力显著性上调(与0·1μmol/ml相比,P<0·05)。同时,高浓度的H2O2对Hela细胞GSNOR活力也有激活作用。结论甲醛可诱导哺乳动物细胞GSNOR的上调,并且这种上调可能与氧化胁迫有关。
Objective To explore the effects of formaldehyde on the activity of GSNO reductase （GSNOR） and the association of oxidative stress. Methods Hela Cells were exposed to different concentrations of liquid formaldehyde and 100mmol/ml H2O2 for 1h, and GSNOR activity was examined in the cellular lysates. Results Formaldehyde could up - regulate GSNOR in a dose - dependent manner, resulting in elevated GSNOR activity with the increase of formaldehyde concentrations, and the increase was statistically significant when the concentration of formaldehyde was up to 1250mmol/ml （ compared with 0mmol/ml, P 〈 0.05 ）. Meanwhile, 100mmol/ml H2O2 also had similar effect on GSNOR activity. Conclusions Formaldehyde could activate GSNOR in mammalian cells, and the activation might be associated with oxidative stress.
出处
《医学研究杂志》
2006年第12期11-13,共3页
Journal of Medical Research
基金
国家自然基金面上项目:非过敏原性空气污染物诱导过敏性哮喘的分子机理研究(No.30570799)
湖北省科技攻关计划项目(No.2005AA307B01)
