摘要
目的探讨鞘内注射芬太尼联合电针对大鼠慢性炎性痛的镇痛效果及相关机制。方法采用完全弗氏佐剂(CFA)关节炎大鼠模型,将30只鞘内置管成功的成年SD雌性大鼠随机分为5组(n=6):对照组(A组,仅予以鞘内置管)、致炎组(B组,鞘内注射生理盐水25μl连续1周,1次／d)、电针组(C组,模型成功后第1天和第4天,采用电针仪电针大鼠“阳陵泉”和“足三里”两穴,强度≤2 mA,频率2／100Hz,时间30min)、芬太尼组(D组,鞘内注射25μl芬太尼,剂量1μg,1次/d,连续1周)和针药合用组(E组,处理与给药方法同C组、D组)。CFA致痛后第4天模型建立成功,开始电针刺激和给药处理。从给药第1天开始,每天9:00以热板法测量大鼠痛敏分数,连续观察7 d;于给药第7天,10％水合氯醛液按375 mg·kg-1腹腔麻醉大鼠,快速取出脊髓L4-6段,以毛细管电泳仪和免疫组化方法检测脊髓(L4-6)谷氨酸(GLU)含量及脊髓背角P物质(SP)表达。结果B组痛敏分数,脊髓(L4-6)谷氨酸含量和患侧腰髓背角SP的表达均比其它组增加(P<0．05);与C组及D组相比,E组痛敏分数,GLU含量和SP表达均降低(P<0．05)。结论鞘内注射芬太尼加强了电针的镇痛作用,针药合用减轻了慢性炎性痛所致的中枢敏化作用。
Objective To investigate the antinociceptive effect of electro-acupuncture combined with intrathecal （IT） fentanyl in rats with chronic inflammation and the possible mechanism.Methods Thirty adult SD rats weighing 200-250 g were randomly divided into 5 groups （ n = 6 each） ： group A normal animal received no treatment; group B arthritic animal received IT normal saline 25 μl once a day for 7 days starting from the 4th day after CFA injection; group C arthritic animal received electro-acupuncture （EA） at ＇yang-ling-quan＇ and ＇zu-san- li＇ for 30 rain （intensity 2 mA, frequency 2/100 Hz） on the 4th and 7th day after CFA injection; group D arthritic animal received IT fentanyl 1 μg in 25 μl once a day for 7 days starting from the 4th day after CFA injection and group E arthritic animal received EA and IT fentanyl as in group C and D. Arthritis was induced by injection of complete Freund＇s adjuvant （CFA） 0.05 ml into the right ankle joint and was confirmed by swelling redness and decreased spontaneous activity of the joint. Latency for removal of left and right hind paw to noxious thermal stimulation was measured in hot plate test every day for 7 days starting from the 4th day after CFA injection, and hyperalgesia score was calculated： hyperalgesia score （second） = left foot latency- right foot latency. The animals were anesthetized with intraperitoneal 10% chloral hydrate and the L4-6 lumbar segment of spinal cord was removed for determination of glutamate content （by capillary electrophoresis） and expression of P substance （by iuununochemistry） in spinal dorsal horn on the 10 th day after CFA injection. Results Hyperalgesia scores and glutamate content and P substance expression in the spinal dorsal horn were significantly higher in group B than in other three groups （group C, D, E） and were significantly lower in group E than in group C and D. Conclusion IT fentanyl can potentiate the antinociceptive effect of EA. EA combined with IT fentanyl attenuates chronic inflammatory pain- induced central sensitization.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2006年第10期944-947,共4页
Chinese Journal of Anesthesiology
基金
天津市自然科学基金资助项目(043608911)
关键词
芬太尼
电针
谷氨酸
P物质
慢性病
疼痛
注射
脊髓
Fentanyl
Electroacupuncture
Glutamic Acid
Substance P
Chronic disease
Pain
Injections,spinal
