摘要
巴西蘑菇深层发酵的菌丝体,用热水提取出水溶性成分(主要是活性多糖)后,残渣用体积分数85%的乙醇提取,对乙醇提取物进行了抑瘤活性的研究。体外细胞培养实验表明,菌丝体醇提物对人肝癌细胞Bel-7402有一定抑制作用,半抑制浓度(IC50)为1507μg/mL。体内则显示出了较强的抑瘤效果,腹腔注射(i.p.)每天10、50、100 mg/kg,对S180荷瘤鼠的瘤重抑制率分别为44.72%、66.50%和70.49%;灌胃(p.o.)每天800 mg/kg,对S180腹水瘤鼠的生命延长率达到52.94%。结果表明:巴西蘑菇深层发酵菌丝体的醇提物具有较强的抑瘤作用,其具体的功效成分值得进一步研究。
The Basidiomycete fungus, Agaricus brasiliensis sp. （Agaricus blazei Murill）, has traditionally been used as a health food for the prevention of cancer. In this study, The antitumor activity of ethanol extracts from cultivated mycelium was investigated. In vitro analysis revealed that it was directly cytotoxic to human hepatoma cells Bel-7402 at dose more than 1000μg/ml and IC50 was about 1 507 μg/mL. In vivo, intraperitoneal administration （i. p. ） of 10- 100 mg/kg/ day to mice bearing Sarcoma 180 significantly inhibit tumor growth and the inhibition rate of tumor weight reached at 44.72% -70.49%. Oral administration （p. o. ） of 800mg/kg/day to mice carrying Sarcoma 180 ascites considerably prolonged their survival time （increase in life span- ILS： 52.94%）, These results suggested that the alcohol-soluble fraction from mycelium of Agaricus brasiliensis sp. exhibit strong antitumor activity.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2006年第6期54-57,共4页
Journal of Food Science and Biotechnology
关键词
巴西蘑菇
菌丝体
醇提物
抑瘤活性
Agaricus brasiliensis sp.
mycelium
ethanol extracts
antitumor effect
