摘要
目的:探讨大鼠心肌梗死(MI)后细胞因子TGF-β1及心室重塑指标动态变化及其与重塑的关系。方法:结扎冠脉左前降支建立心肌梗死(MI)模型并设立假手术对照组。术后3天、1周、4周和24周检测血流动力学和组织形态学及胚胎基因(β肌球蛋白重链),Ⅰ、Ⅲ型胶原和TGF-β1的mRNA水平。结果:与假手术组相比,MI组血流动力学改变明显。心肌梗死后第3天胚胎基因β-MHC、胶原、TGF-β1都上升,一直持续到第4周。梗死区Ⅰ、Ⅲ型胶原在第4周仍然比非梗死区高即使在第24周非梗死区的胶原水平仍然高于假手术组。TGF-β1在第1周达到高峰后逐渐下降到第24周时非梗死区与假手术组相比有统计学差异(P<0·01),相关性分析提示梗死区TGF-β1与Ⅰ、Ⅲ型胶原、β-MHC存在相关;非梗死区TGF-β1与Ⅰ、Ⅲ型胶原存在相关。结论:TGF-β参与心室重塑病理生理过程并且可能是心室重塑的病因之一。
Objective：To analyze the dynamic change of TGF-β1 in the ventricular remodeling process of rat after myocardial infarction and reveal the relationship between them. Methods：MI model was achieved by ligating the left anterior descending coronary artery and sham operated rats were designated to control groups. The mRNAs of Collagen type I ,Collagen type Ill ,TGF-β1 ,fetal gene （ beta myosin heavy chain） and hemodynamics change were detected at 3 days, 1,4,24 weeks as well as the histomorphological observa- tion. Results：The hemodynamics differences were remarkable between the MI groups and sham groups. Beta-MHC, Collagen type Ⅰ , Collagen type Ⅲ ,TGF-β1 mRNA levels were elevated at 3 days,and were persistent for4 weeks expression of collagen type Ⅰ and Ⅲ was predominant in the infracted. Zone than that of in non-infarcted zone while it was higher than sham groups in the non-infarcted zone at 24 weeks. Compared to the sham operated groups, the levels of TGF-β1 reached their peak at 4 weeks, and then decreased gradually. TGF-β1 was significantly higher than the sham groups in the non-infracted zone in the 24 weeks（ P 〈 0. 01 ）. Correlation analysis of the mRNA expression indicated that fetal gene β-MHC and α- MHC, collagen subtype Ⅰ and Ⅲ ,were correlated positively significantly to pro-inflammatory factor（TGF-β1 ） wherever in infracted and non-infracted zone（P 〈0.01 ）. Conduslon：TGF-β1 participated in the myocardial remodeling of myocard/al infarction at least, which is the one of the possible pathogenesis.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2006年第12期1075-1078,共4页
Chinese Journal of Immunology
基金
国家自然科学基金资助项目(编号:30370574)
