摘要
应用CTAB法、改良CTAB法、改良CTAB-高盐沉淀法对毛竹基因组DNA进行了提取,并用紫外分光光度计(UV3300)、琼脂糖凝胶电泳和PCR分析对提取的DNA进行了检测,对DNA的产量、质量、PCR效果等方面进行了综合比较。结果表明:改良CTAB-高盐沉淀法是提取高质量毛竹基因组DNA的较好方法。
CTAB method, improved CTAB method, improved CTAB-high salt precipitation method were used to extract genomic DNA from the leaves of Ph. edulis. The DNA samples obtained were tested by UV spectrophotometer 3 300, agarose gel electrophoresis and PCR method. The differences in yield, quality and PCR result of the obtained DNA were compared. The results revealed that improved CTAB-high salt precipitation method was an inexpensive and reliable method for the genomic DNA extraction of Ph. edulis.
出处
《林业科学研究》
CSCD
北大核心
2006年第6期725-728,共4页
Forest Research
基金
国际竹藤网络中心青年基金"花雄性不育突变体的鉴定及其调控基因的初步定位研究"
国家林业局948项目(2005-4-38)
