摘要
以莎能奶山羊(Saanen dairy goat)转基因(GFP)成纤维细胞为核供体细胞,以普通山羊卵母细胞为受体细胞进行了核移植,试验中对转基因核移植胚胎的电融合条件进行了优化,发现对于莎能奶山羊转基因(GFP)重构胚胎的处理,电融合时方案B(1.5kV/cm、30μs/次、间隔1s)电融合参数最适合用于莎能奶羊的转基因(GFP)核移植胚胎,其重构卵电融合率为89.4%,重构胚分裂率为68.0%。
In this study using the transgenic （GFP） fibroblasts of Saanen dairy goat as donor cells and using the abattoir-derived oocytes of normal goat as recipient cells the nuclear transfer experiments had been processed. During experiments the optimization of electricfusion experiment parameters of transgenic nuclear transfer embryos had been done. For the treatment of transgenic reconstruction embryos of Saanen dairy goat, the electricfusion parameter of group B in experiment plans （1.5kV/cm for 30 μs and pulse interval for ls） is most suitable for the transgenic reconstruction embryos of Saanen dairy goat, the electricfusion rate of 89.4 % for cellcytoplast complexes and the cleavage rate of 68.0% for reconstructed embryos have been obtained.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2006年第12期1368-1372,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金项目(30471242)
中科院2002年第2期"百人计划"项目
关键词
转基因
核移植
电融合
重构胚胎
体细胞克隆
transgenic
nuclear transfer
electrofusion
reconstruction embryo
somatic cloning
