摘要
研究了嗜酸乳杆菌NCFM菌株经胶囊化处理后对逆境的抵抗能力。结果表明,经pH值为2.5的酸处理后,微胶囊化菌株的活菌数(6.4×108 mL-1)、酸度(95oT)和!-半乳糖苷酶活力(0.127 mmol/g.min),明显高于对照菌株(P<0.01)的活菌数(2.3×108 mL-1)、酸度(56 oT)和!-半乳糖苷酶活力(0.078 mmol/g.min);经质量浓度为14 g/L的胃蛋白酶处理后,与对照菌株的活菌数(7.8×108 mL-1)、酸度(70 oT)和!-半乳糖苷酶活力(0.01 mmol/g.min)相比,微胶囊化菌株的活菌数、酸度、!-半乳糖苷酶活力明显提高(P<0.01),分别为1.41×109 mL-1,119 oT和0.075 mmol/g.min;经质量分数为0.4%胆盐处理后,微胶囊化菌株的活菌数(4.8×108 mL-1)、酸度(98 oT)和!-半乳糖苷酶活力(0.97 mmol/g.min),明显高于对照菌株(P<0.01)的活菌数(2.9×108 mL-1)、酸度(52 oT)和!-半乳糖苷酶活力(0.43 mmol/g.min);经质量浓度为3 g/L胰蛋白酶处理后,与对照菌株的活菌数(0.2×107 mL-1)、酸度(48 oT)和!-半乳糖苷酶活力(0.017 mmol/g.min)相比,微胶囊化菌株的活菌数、酸度、!-半乳糖苷酶活力显著提高(P<0.01),分别为3.5×108 mL-1,90.8 oT和0.1 mmol/g.min;经质量分数为18%的NaCl溶液处理后,微胶囊化菌株的活菌数(11.7×108 mL-1)、酸度(102 oT)和!-半乳糖苷酶活力(0.52 mmol/g.min),明显高于对照菌株(P<0.01)的活菌数(0.37×108 mL-1)、酸度(38 oT)和!-半乳糖苷酶活力(0.18 mmol/g.min)。显然,微胶囊化处理能明显提高嗜酸乳杆菌在极端环境下的存活能力。
Microencapsulation of Lactobacillus acidophilus strain NCFM subjected to extreme environments was studied. The viability of the microencapsulated strain NCFM treated in pH 2.5 was significantly improved compared to the control （P〈0.01）, The viable cell counts, titratable acidity and β-Galactosidase level with microencapsulated cells were 6.4×10^8 mL^-1, 95°T and 0.127 mmol/（g·min） respectively; whereas those were 2.3×10^8 mL^-1, 56 °T and 0.078 mmol/（g·min） in non-microencapsulated cells treated as the control, respectively ; Exposure strain NCFM to 14 g/L pepsin solution, It was seen that compared to the non-microencapsulated cells, the viable cell counts, titratable acidity and β-Galactosidase level with the micro-encapsulated cells were 1.41×10^9mL^-1,119°T and 0.075 mmol/（g·min）, respectively, whereas the viable cell counts, titratable acidity and β-Galactosidase level of the control treatment were 7.8×10^8 mL^-1, 70°T and 0.01 mmol/（g·min） respectively. When treated in 0.4% bile salt, the viable cell counts, titratable acidity and β-Galactosidase level with microencapsulated cells were higher than the control （P〈0.01）, reaching 4.8×10^8 mL^-1, 98°T and 0.97 mmol/g·min, respectively, whereas the control were 2.9×10^8 mL^-1,52 °T and 0.43 mmol/g·min respectively. Exposure of strain NCFM to 3 g/L trypsin solution showed that compared to the non-microencapsulated cells, the viable cell counts, titratable acidity and the β-Galactosidase level with the micro-encapsulated cells were 3.5 × 10^8 mL^-1, 90.8°T and 0.1 mmol/g·min respectively. Whereas the viable cell counts, titratable acidity and β-Galactosidase level of the control treatment were 0.2×10^7 mL^-1, 48°T and 0.017 mmol/（g·min） respectively. When treated in 18% NaCl solution, the viable cell counts, titratable acidity and β-Galactosidase level with microencapsulated cells were higher than the control （P〈0.01）, up to 11.7 × 10^8 mL^-1, 102°T and 0.52 mmol/ （g·min） respectively, whereas the control were 0.37×10^8 mL^-1, 38 °T and 0.18 mmol/（g·min） respectively. In short, it was clear that use of microencapsulation presented in our study to treat L. acidophilus promoted the survival of this organism subjected to extreme environments.
出处
《中国乳品工业》
CAS
北大核心
2006年第12期7-12,共6页
China Dairy Industry
关键词
嗜酸乳杆菌
微胶囊
逆境
存活力
Lactobacillus acidophilus
microencapsttlation
extreme environments
viability
