摘要
目的:探讨艾炷灸贴预处理对佐剂性关节炎(AA)大鼠早期与继发期血清炎性因子和激素的影响及保护机制。方法:将40只Wistar大鼠随机分为正常组、模型早期组和继发组、预灸贴早期组和继发组。于造模前用中药膜剂贴敷大鼠“大椎”穴后加艾炷灸,采用弗氏完全佐剂制备大鼠模型,用放射免疫法测定各组大鼠血清白细胞介素-6(IL-6)、睾酮(T)、生长激素(GH)及皮质醇(CORT)的含量。结果:血清IL-6和GH含量造模后均明显升高(P<0.01);与同期模型组相比,预灸贴继发组均明显下降(P<0.05)。血清T含量造模后早期组明显降低(P<0.05),继发组变化不大;预灸贴各组较同期模型组均显著升高(P<0.01,0.05)。血清CORT含量造模后显著升高(均P<0.01),而预灸贴继发组与正常组比无明显差异(P>0.05)。结论:艾炷灸贴预处理可减轻随后佐剂性关节炎大鼠原发足局部病理损伤,降低血清炎性因子IL-6的水平,对血清T、GH及CORT等内分泌激素的紊乱状态具有良性调节作用。
Objective： To observe the effects of acupoint-application and moxibustion pretreatment on serum inflammatorycytokine and hormones of rats with adjuvant arthritis at early and secondary stages. Methods： Forty Wistar rats were randomly divided into normal, early-stage model, secondary-stage model groups, early-stage pretreatment and secondary-stage pretreatment groups. Arthritis model was established by injecting Freund＇s complete adjuvant （FCA, 0. 1 mL） into the rat＇s right hindpaw. After removing the hair and before injecting FCA respectively, a plaster made of Herba Epimedii, etc. and an ignited moxa cone （placed on the plaster） were applied to ＂Dazhui＂ （GV 14）, once every other day, 8 times altogether. Rats of the 4 earlystage and secondary-stage groups were killed on the 3^rd day and 16^th day after injection of FCA respectively, and those of normal group also killed for collecting blood samples. The contents of serum IL-6, testosterone （T）, growth hormone （GH） and cortisol （CORT） were assayed respectively with radioimmunoassay according to the instructions of reagent kits. Results： Compared with normal group, the contents of serum IL-6, GH and CORT of early-stage-model and secondary-stage-model groups increased significantly （P〈0.01）, and those of early-stage pretreatment groups, and serum T and GH of secondary-stage pretreatment groups also increased remarkably （ P〈0.05, 0.01 ） ; but T level of early-stage model group decreased significantly （ P〈O. 05） ; while in comparison with the corre-sponding model groups, the contents of T in early-stage and secondary-stage pretreatment groups increased considerably （P〈0.05,0.01 ） ; and those of serum IL-6 and GH lowered markedly （P〈0.05） ; no significant differences were found among the 2 model and 2 pretreatment groups （P〉0.05）. Conclusion： Preconditioning of acupoint-application and moxibustion at GV 14 can effectively suppress arthritis induced increase of serum IL-6 and GH levels and decrease of T to modulate inflammatory cytokine and endocrine hormones, playing a protective role in adjuvant arthritis rats.
出处
《针刺研究》
CAS
CSCD
2006年第6期330-332,341,共4页
Acupuncture Research
基金
国家自然科学基金(No.90209026)
北京市自然科学基金(No.7052037)项目资助
关键词
艾炷灸贴预处理
佐剂性关节炎
血清IL-6
内分泌激素
Acupoint-application and moxibustion-preconditioning Adjuvant arthritis Serum IL-6 Endocrine hormones
