摘要
目的观察胆碱对大鼠急性心肌缺血的作用,并探讨其作用机制。方法以结扎大鼠左冠状动脉前降支造成急性心肌缺血模型,给予M受体激动剂胆碱和M3受体阻断剂4DAMP进行干预。观察大鼠急性心肌缺血模型心律失常发生情况和心肌细胞L型钙通道蛋白mRNA的表达。结果①胆碱可降低大鼠急性心肌缺血模型的心律失常发生率(P<0.05);②胆碱能够使L型钙通道mRNA表达增加;③M3受体阻断剂4DAMP可逆转胆碱的上述作用(P<0.05)。结论胆碱通过激活M3受体降低了急性心肌缺血大鼠心律失常的发生率。
Objective To observe the effects of choline on acute myocardial ischemia and explore the reasonable mechanism. Methods Construct a model of acute myocardial ischemia in rots by ligating the descending anterior branch of left coronary artery. For the intervention, M receptor agonist, choline and its antagonist choline were used. The incidence of arrhythmia and the mRNA expression of L type Ca^2 ＋ channel protein were investigated. Results ①Choline decreased the incidence of arrhythmia on the model of acute myocardial isehemia in rats;②Choline increased the mRNA expression of L type Ca^2＋ channel protein;③M3 receptor antagonist,4DAMP may reverse the effects of choline on the model of acute myocardial isehemia in rats completely. Conclusion The incidence of arrhythmia in acute myocardial isehemia could be decreased by choline through activating M3 receptor.
出处
《哈尔滨医科大学学报》
CAS
北大核心
2006年第6期446-449,共4页
Journal of Harbin Medical University
