摘要
目的:研究雷公藤甲素对人肺腺癌A549细胞增殖和凋亡的影响。方法:采用MTT实验检测雷公藤甲素对体外培养的人肺腺癌A549细胞增殖的影响,运用流式细胞术、基因组DNA电泳观察凋亡特征性“梯状”条带检测细胞凋亡,采用caspase活性定量检测试剂盒分析caspase-9,3的活化。结果:雷公藤甲素对体外培养的A549细胞具有明显的抑制作用。流式细胞仪检测显示雷公藤甲素可以显著诱导A549细胞凋亡,DNA电泳显示雷公藤甲素作用于A549细胞后出现凋亡细胞特有的DNA阶梯状条带。雷公藤甲素作用后A549细胞caspase-9,caspase-3活性明显升高。结论:雷公藤甲素可能通过线粒体信号通路抑制肺腺癌A549细胞的生长并诱导其凋亡。
AIM： To study the effect of triptolide on cell proliferation and apoptosis in As49 human lung cancer cell. METHODS： The effect of triptolide on ceil viability in A549 cell was evaluated by MTT assay. Cell apoptosis was observed by flow cytometry and DNA ladder analysis. Activities of caspase-9 and caspase-3 were assayed with caspase colorimetric assay kit. RESULTS： Triptolide （ eg., 0.5 -4.0μg·ml^-1 ） inhibited ceil growth and induced apoptosis in a dose-dependent way at 24 h in A549 cells. The activation of caspase-9 and caspase-3 was significantly increased at 24 h in A549 cells when exposed to triptolide （eg., 0.5 -4.0 μg·ml^-1 ）. CONCLUSION： Triptolide induces apoptosis in A549 cells partly through mitochondrial signal transduction pathway.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2006年第11期1275-1278,共4页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
湖南省科技厅基金项目(№05SK3084)
衡阳市科委项目(№2005SK01-017)
