摘要
目的通过合成所有细菌共有的16SrRNA基因高度保守区引物,进行PCR扩增,探讨败血症的快速诊断方法。方法用PCR技术扩增实验室保留株10株的16SrRNA基因,以人类基因组DNA、HBV-DNA和白色念珠菌为对照,检测该方法的特异性;采用倍比稀释法进行该方法的灵敏度检测。结果对所测细菌株均获得920bp扩增产物,而与人基因组DNA、HBV-DNA和白色念珠菌无交叉反应;PCR最低能检测1.5×106/L大肠杆菌DNA。结论PCR检测方法是一种极有应用价值的用于败血症早期诊断方法。
Objective To explore a method for rapid diagnosis of septicemia, a experimental procedure based upon polymerase chain reaction （PCR） amplification using a primer pair for highly conserved segment of 16SrRNA gene. Methods 16SrRNA gene of ten bacterial species were amplified with PCR, by using human genome DNA、HBV - DNA and candida albicans as comparison; The sensitivity test was done by the method of gradual dilution. Results The bacterial species were amplified and the products were 920bp in length, but human genome DNA、HBV - DNA and candida albicans showed no amplification products. Sensitivity test showed that it could detect as low as 1.5×10^6/L of E. coli DNA. Conclusions PCR technology is an exteremely value detection method for diagnosis of septicemia.
出处
《医学研究杂志》
2006年第11期54-56,共3页
Journal of Medical Research
