摘要
目的探讨细胞间黏附分子-1(ICAM-1)基因K469E多态性在冠心病及正常人群中的分布,初步分析其基因型及血清水平与冠心病的关系。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术和DNA序列测定法,检测了225例冠心病患者和230例对照者的ICAM-1基因K469E多态性,并用酶联免疫吸附试验检测了ICAM-1的血清水平。结果冠心病组血清ICAM-1水平显著高于对照组(P<0.01),ICAM-1基因型及等位基因的分布频率在冠心病组和对照组间比较差异具有显著性(P<0.05),K等位基因携带者患冠心病的相对风险度是E等位基因的1.430倍(与对照组相比),而患心肌梗死的相对风险度是1.816倍(与心绞痛组相比)。结论ICAM-1基因K469E多态性与冠心病的发生、发展及该疾病的严重程度密切相关,其中K等位基因可能是冠心病发病的遗传易感基因。
Objective ：To study the polymorphism of intercellular adhesion molecule-1 （ ICAM-1 ） gene K469E in coronary heart disease（CHD） and healthy population, and to analyze association the serum level and genotypes of ICAM-1 with CHD. Methods： Using the PCR-RFLP method, the ICAM-1 gene K469E polymorphism in 225 CHD patients and 230 healthy controls were detected, and the serum level of ICAM-1 was determined by enzyme-linked immunosorbent assay（ELISA）. Results：The CHD group showed significantly higher serum levels of ICAM-1 than control group（ P 〈 0. 01 ）, the ICAM-1 gene K469E polymorphism was significantly difference between CHD group and control group. The relative risk suffered from CHD of E allele was 1. 430 times of the K allele. The relative risk suffered from acute myocardial infarction（AMI） of E ＇allele was 1. 816 times of the K allele. Conclusion：The ICAM-1 gene K469E polymorphism is significantly associated with the occurrence, development and the severity of CHD, and K allele may be a genetic risk factor of CHD.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2006年第11期1056-1059,共4页
Chinese Journal of Immunology
基金
广西自然科学基金资助项目(0499006)
右江民族医学院科研基金资助项目(No.03001)
