摘要
采用组织化学方法对长牡蛎(Crassostrea gigas)胚胎和早期幼虫中的非特异性酯酶(non-specific esterase,NSE)和过氧化物酶(peroxidase,POD)进行定位研究,以探讨贝类胚胎和幼虫发育过程中的免疫防御机制。组织化学显示,NSE和POD均广泛存在于长牡蛎的胚胎和幼虫中,自卵细胞开始就已存在。NSE和POD在各个发育时期分布和含量不同,精子呈极弱的NSE和POD阳性;未受精的卵呈强阳性,但酶在细胞内的分布不均匀,核区颜色较浅;2至32细胞期细胞内酶的分布较均匀;桑葚期、囊胚期、原肠期以及担轮幼虫和早期面盘幼虫期不同细胞间酶的强弱有差异。在胚胎发育前期显色较深,呈强阳性,表明此2种酶的活性较高;发育至后期颜色变浅,呈弱阳性,表明酶的活性降低;幼虫期酶活性有增强的趋势。
The non-specific esterase（NSE） and peroxidase（POD） on embryo and early larva of Crassostrea gigas were studied by histochemical localization method. The aim of the present study was to probe into the immune mechanism in the development process of mollusk＇ s embryo and larva. The results showed that both NSE and POD existed in the egg cells, embryo or early larva of C. gigas. In different development stages the distributions and activities of NSE and POD were different. Unfertilized egg cell presented strong positive reactions of NSE and POD,with the distribution of the enzymes in the cell ununiform and the nucleus＇ colour lighter than other areas. The distribution of NSE and POD in a cell were relatively uniform from 2-cell stage to 32-cell stage. NSE and POD presented different activities in different stages of morula, blastula, gastrula and trochophore larva. On the other hand, the spermatozoon presented extremely weak NSE and POD positive reaction. The presented colour in cells was lighter and lighter during the period of embryo development, indicating the activities of NSE and POD were getting lower and lower. It suggests that these two kinds of enzymes may come from mother＇s body, the enzymes are accumulated in the egg cytoplasm in the forming process of the egg cell. In the larva stage the enzymes activities tend to be stronger again.
出处
《中国水产科学》
CAS
CSCD
北大核心
2006年第6期897-901,共5页
Journal of Fishery Sciences of China
基金
国家"973"计划资助项目(G1999012005)
关键词
长牡蛎
胚胎
幼虫
非特异性酯酶
过氧化物酶
组织化学
Crassostrea gigas
embryo
larva
non-specific esterase
peroxidase
histochemistry
