摘要
在基础饲料中分别添加硒至0．0mg·kg^-1、0．2mg·kg^-1、0．4mg·kg^-1、0．6mg·kg^-1、0．8mg·kg^-1和1．0mg·kg^-1，分别饲喂鲈鱼（Lateolabrax japonicus）10周，观察其对鲈鱼生长性能、肝脏及血液中谷胱甘肽过氧化物酶（GSH-Px）、肝脏谷胱甘肽还原酶（GR）、肝脏谷胱甘肽转移酶（GST）活性的影响。实验鲈鱼初始体质量为（26．5±1．02）g，实验结束后饥饿24h再进行测定。结果显示，SGR的最大值及FCR的最小值均出现在硒水平0．4mg·kg^-1饲料组，该组鱼体蛋白质含量也显著高于其他各组（P〈0．05）；硒水平为0-0．4mg·kg^-1饲料组鲈鱼的肝脏、血清中GSH-Px活性随着硒添加量的增加而提高，饲料硒水平为0．4mg·kg^-1时达到最高，当硒的添加水平继续提高到0．8～1．0mg·kg^-1时，鲈鱼的肝脏、血清中GSH-Px活性均显著下降（P〈0．05）；肝组织GR活性在饲料硒水平达到0．4mg·kg^-1时达到最高值，饲料硒水平为0．6mg·kg^-1时，GR的活性显著下降（P〈0．05）。建议鲈鱼饲料硒的适宜添加水平为0．4mg·kg^-1。
Six levels of dietary Se were designed at 0.0 mg·kg^-1,0.2 mg·kg^-1,0.4 mg·kg^-1, 0.6 mg·kg^-1,0.8 mg·kg^-1 and 1.0 mg·kg^-1 respectively and fed to juvenile Japanese seabass（ Lateolabrazc japonicus ） for 10 weeks. The initial body weight of seabass was （26.5 ＋ 1.02） g. After 10 weeks experiment the fish were deprived of food for 24 h and then the GSH-Px activities in serum and liver, GR and GST activities in liver were analyzed and the growth performances were evaluated. The results indicated that in the treatment with diary Se at 0.4 mg·kg^-1, seabass got the highest SGR and the lowest FCR, and the protein content in fish body was significantly higher than those in other treatments（P 〈 0.05）. The GSH-Px activities in serum and liver increased as dietary Se level raising from 0 to 0.4 mg·kg^-1 and reached peak with Se level at 0.4 mg·kg^-1, but when dietary Se level continued to raise from 0.8 mg·kg^-1 to 1.2 mg·kg^-1, the GSH-Px activities in serum decreased significantly（P 〈 0.05）. The highest GR activity in liver was observed in the seabass fed diet of Se level 0.4 mg· kg^- 1, and GR activities in liver decreased when diary Se level was 0.6 mg· kg^- 1.
出处
《中国水产科学》
CAS
CSCD
北大核心
2006年第6期1017-1022,共6页
Journal of Fishery Sciences of China
基金
国家"十五"攻关项目(2004BA526B06)
关键词
鲈鱼
硒
生长性能
酶活性
Lateolabrax japonicus
Se
growth performance
enzym activity
