摘要
选生长健壮的大花蕙兰盆栽大苗,取不同月份假鳞茎上新生的侧芽,研究其组培快繁技术。结果表明:M S+6-BA 5.0 m g/L+NAA 2.0 m g/L是原球茎诱导最佳培养基,12月份和1月份的芽成苗率最高;M S+6-BA0.2 m g/L+香蕉200 g/L+蔗糖20 g/L培养基原球茎增殖率最高,井字形切割可使继代周期缩短,增殖率高;最佳生根壮苗培养基是1/2 M S+NAA 0.7 m g/L;生根苗有3条~4条根,根长0.5 cm^1 cm时,先在温室炼苗1周,再打开瓶盖适应环境2 d^3 d后即可移栽,约6月至8月后,可移栽于10 cm营养杯单株种植。
Using the tender buds in pseudobulb of vigorous pot-seedling of Cymbidium grandiforium as micropropagation materials, the results were that, protocorm was induced on the culture medium MS＋ 6-BA 5.0 mg/L＋ NAA 2. 0mg/L, the cultured seedling mainly came from the tender buds in January and December. Proliferate rate of protocorm was the best on the culture medium MS＋6-BA 0. 2mg/L＋banana flesh 200 g/L＋sucrose 20g/L. #- shape cut will shorten secondary culture period and increase multiplication rate. The optimal culture medium for rooting is 1/2 MS＋NAA 0. 7 mg/L. Every cultured seedling has 3-4 roots （0. 5-1cm in length）. After adapting to greenhouse for a week in the culture-pot with lid, then for 2-3 days uncover lid, these seedling was transplanted, after 6-8 months, they will be again transplanted in seedling-cup of 10cm size.
出处
《山西林业科技》
2006年第4期38-39,共2页
Shanxi Forestry Science and Technology
关键词
大花蕙兰
继代培养
生根培养
移栽基质
Cymbidium grandiforium
secondary culture
generating root culture
substratum of transplanting
