摘要
目的研究微血管密度在胃癌癌变多阶段组织中的表达及其意义。方法标本取自中山大学中山医学院病理学教研室保存的2000年6月至2004年2月的病理组织蜡块,其中慢性胃炎56例、慢性萎缩性胃炎50例、肠化生59例、不典型增生61例;在胃癌组织标本中,无淋巴结转移胃癌组织55例,有淋巴结转移胃癌组织57例。免疫组织化学Envision法显示胃癌癌变多阶段组织中CD34的表达。结果Envision方法检测微血管密度的结果(个/高倍视野,用x-±s表示):慢性胃炎组为13±10;慢性萎缩性胃炎组为11±7;肠化生组为13±9;不典型增生组为17±11;无淋巴转移胃癌组为27±11;有淋巴转移胃癌组为28±10。微血管密度在慢性胃炎组、萎缩性胃炎组、肠化生组组间比较,差异无统计学意义(均P>0·05);萎缩性胃炎组、肠化生组、不典型增生组组间比较,差异无统计学意义(均P>0·05);慢性胃炎组低于不典型增生组(P<0·05);慢性胃炎、萎缩性胃炎、肠化生、不典型增生各组均低于无淋巴转移胃癌组织、有淋巴转移胃癌组(均P<0·05);在胃癌组中,无淋巴转移胃癌组织与有淋巴转移胃癌组织组间比较,差异无统计学意义(P>0·05),其组织学病理程度高中分化组、与低分化组间差异也无统计学意义(P>0·05)。结论微血管密度在胃癌癌变多阶段组织中增加与胃癌的发生有关,与淋巴结转移无关。
Objective To investigate the expression of microvessel density （MVD） in multistep tissue of gastric carcinoma and CD34 in the multistep tissue of gastric carcinogens. Methods 277 specimens of gastric diseases, 56 specimens of chronic gastritis, 50 specimens of chronic atrophic gastritis, 59 specimens of intestinal metaplasia, 61 specimens of displasia, 57 specimens of gastric carcinoma with lymph node metastases, and 55 specimens of gastric carcinoma without lymph node metastases, obtained from pathological department, Sun Yat-sen university, underwent pathological examination and immunohitocbemistry to detect the expression of CD34 antigen that is expressed only in the endothelial cells in tumor. Results The mean MVD was 13 ± 10 in the specimens of chronic gastritis, 11 ± 7 in the chronic atrophic gastritis group, 13 ±9 in the intestinal metaplasia group, 17 ± 10 in the displasia group, 27 ±11 in the gastric carcinoma without lymph node metastases group, and 28 ± 10 in the gastric carcinoma with lymph node metastases group. There were not significant differences in MVD among the chronic gastritis group, chronic atrophic gastritis group, and intestinal metaplasia group （ all P 〉 0.05 ）, there was a significant difference in MVD between the chronic gastritis group and displasia group （ P 〈 0.05 ）. In the specimens of gastric carcinoma, there were no significant difference in MVD between those with and without lymph node metastases （P 〉 0.05 ）, and between those of high and low differentiation degrees （ both P 〈 0.05 ）. Condttsion The increase of expression of CD34 in the multistage tissues of gastric carcinoma is associated with the genesis of gastric cancer, and not be related te the gastric carcinoma metastases.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2006年第48期3405-3408,共4页
National Medical Journal of China
基金
国家自然科学基金资助项目(30670949
30671904)
新世纪优秀人才支持计划基金资助项目(NCET-04-0785)
中国博士后基金资助项目(2004035181)
广东省自然科学基金资助项目(4009350)
关键词
免疫组织化学
胃肿瘤
肿瘤转移
抗原
CD34
微血管密度
Immunohisteebemistry
Stomach neoplasms
Neoplasm metastasis
Antigens CD34
Microvessel density
