摘要
目的研究姜黄素对前列腺癌细胞LNCaP凋亡的影响和对maspin基因表达的调控作用。方法利用不同浓度的姜黄素处理前列腺癌细胞LNCaP,MTT法和DNA电泳检测不同时相LNCaP细胞的活性和凋亡情况,RT-PCR和W estern b lotting检测转录水平和翻译水平maspin基因的表达情况。将包含maspin5′侧启动子区847 bp(-764^+83)DNA的荧光素酶表达载体pGL3-maspin瞬时转染LNCaP,姜黄素作用细胞48 h后,应用荧光素酶测定系统检测maspin启动子的表达活性作用。结果姜黄素抑制LNCaP细胞的生长活性,诱导LNCaP细胞的凋亡,增强LNCaP细胞中maspin基因的表达。结论姜黄素增强LNCaP细胞中maspin基因的表达是通过促进启动子的转录活性起作用的。
Aim To study the effect of curcumin on the apoptosis of prostate cancer cell line LNCaP and regulation of expression of maspin gene. Methods MTT and DNA electrophoresis were used to examine the cell growth and apoptosis of prostate cancer cell line LNCaP after treated with different doses of curcumin. The expression of maspin gene at transcription level and translation level was also detected by RT-PCR and Western blotting, pGL3-maspin luciferase expression vector, containing 847 bp （ -764 - ＋83） DNA of maspin gene 5＇ promoter region, was transient transfeeted into LNCaP cell. Through detecting the activity of luciferase, the effect of curcumin on the promoter of maspin was studied. Results Curcumin inhibited cell growth, induced the apoptosis and enhanced the expression of maspin gene in LNCaP cells. Conclusion Curcumin up-regulated expression of maspin gene in LNCaP cells through enhancing the transcription activity of promoter of maspin gene.
出处
《药学学报》
CAS
CSCD
北大核心
2006年第12期1152-1156,共5页
Acta Pharmaceutica Sinica
基金
国家自然科学基金资助项目(30371564
30470820).
