摘要
利用自制高压静电场装置,对桔青霉菌进行不同场强度、时间及温度的处理来考察高压静电场对其孢子萌发率的作用效果;用典型作用参数处理观察青霉菌芽管长度、接种于健果上的菌斑大小及孢子形态来进一步验证对萌发率作用结果及作用机理的探讨。结果表明,高压静电场对青霉菌孢子的萌发具有促进和抑制两种完全不同的作用。场强小于40000V/m和处理时间小于20min能促进孢子萌发,场强大于62000V/m和处理时间超过60min能抑制孢子萌发,中间剂量的处理效果依据不同的处理而有所变化。温度对电场处理效果有着显著的影响,当温度升高到50℃,高剂量的场强处理可以完全抑制青霉菌孢子萌发。高压静电场对青霉菌的芽管长度、桔果上的菌斑大小的作用有着相似的结果。62000V/m、60min的处理使青霉菌的孢子形态结构受损,导致其孢子失去萌发能力。结果表明高压静电场为柑橘类的果蔬保鲜提供了一条物理处理新途径。
The research based upon biological effect of electrostatic and magnetic field. In the study, the effects and mechanism of high voltage electrostatic field（HVEF） on P. italicum was researched by changing the two parameters of treatment time and temperature in order to afford useful data for future investigating biological effect of HVEF and its utilization to preserve fruit. The main results of this research are the following： HVEF positively increases the germination rate of P.itatlicum within the 40000V/m and 20min treatment time. However, HVEF distinctly restrains spores from germinating over 62000V/m intensity and 60min treatment time. The influence of treatment temperature on the effect of HVEF upon P. italicum is observable, especially the treatment of 50℃, 62000V/m and 60min completely restrains spores germinating. Colony diameter on PDA and circle of spread disease oranges identify the changing tendency of germination rate of P.italicum with typical treatments. P. italicum spore is terribly destroyed with the treatment of 62000V/m and 60min. These results indicate that HVEF of high treating dose can be used to store and preserve fruits.
出处
《食品科技》
CAS
北大核心
2006年第12期61-65,共5页
Food Science and Technology
关键词
高压静电场
微生物效应
桔青霉菌
high voltage electrostatic field
biological effect
P. italicum
