摘要
目的：本研究利用组织芯片技术高通量地研究Fascin蛋白在食管癌中的表达。方法：采用56例食管癌病人的病理蜡块制作组织芯片,应用免疫组化的方法检测Fascin在食管癌组织中的表达情况。同时对8例食管癌标本的冰冻切片显微切割“纯”癌和非癌组织,进行蛋白印迹研究。结果：在55例可提供信息的病例中,总体上72．7%的肿瘤中为阳性染色。其中高分化阳性率为95．2%,中分化为75%,低分化为35．7%,Fascin表达与食管癌分化有关(P＜0．001)。蛋白印迹结果也证实在分化较好的食管癌中Fascin为高表达。结论：利用组织芯片可以应用于回顾性Fascin表达的研究,研究中发现食管癌中Fascin的表达与组织分化有关。该试验方法简便,易于在临床推广。
Objective：Fascin is a prognostic factor for Esophageal Scluamous Cell Carcinoma （ESCC）. The aim of this study was to explore the expression of Fascin in ESCC by tissue array analysis. Methods： Tissues from 56 ESCC patients were arranged into one wax block by tissue arrayer. Immunohistochernistry was applied to evaluate the expression of Fascin. Mean while, 8 paired tumor and non-cancerous normal tissues were cryosectioned and ＂pure＂ cancer and normal tissue were separated by laser captured rnicrodissection. Expression of Fascin in these captured tissues was validated by western blot. Results： In 55 informative cases, overall 72. 7% tumor was Fascin positive. With regard to tumor differentiation, Fascin positive rate was 95.2% in well differentiated tumor, while 75% in intermediate differentiated tumor and 35.7% in lower differentiated tumor. And the expression related to tumor differentiation （P〈0. 001）. Results of western-blot proved high expression of Fascin in well to intermediate differentiated tumors. Conclusion：Tissue array can be applied to retro-respectively evaluate the expression of protein Fascin. In this study, Fascin expression was found tumor differentiation related in ESCC.
出处
《中国临床医学》
北大核心
2006年第6期924-926,共3页
Chinese Journal of Clinical Medicine
基金
国家自然科学基金资助项目(批准号:30471677)
关键词
食管癌
组织芯片
FASCIN
蛋白印迹
Esophageal neoplasms
Tissue array analysis
Fascin
Western blotting
