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新生大鼠心肌细胞培养方法的改进 被引量:13

Isolation and culture of cardiomyocytes in neonatal rat
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摘要 目的:建立便捷,成熟的新生大鼠心肌细胞体外培养方法。方法:本实验对常用的培养方法加以改进.采用胰蛋白酶及Ⅰ型、Ⅱ型胶原酶共同消化法,分离新生大鼠心肌细胞,并对体外培养的心肌细胞的存活率、活力及纯度进行鉴定。结果:分离的心肌细胞存活率为96%,培养3d后有60-80%的细胞开始搏动,搏动频率平均50次/min,心肌细胞纯度在75%以上。结论:本实验建立的新生大鼠心肌细胞体外培养方法是成熟,可靠的。 Objective: To establish a convenient and mature method of isolation and culture of cardiomyocytes in neonatal rat. Methods: Cardiomyocytes of neonatal rat were isolated in trypsin and collagenase. Then the livability, energy and the purity of cardiomyocytes were identified. Results: The livability of cardiomyocytes was 96 % ; 60%- 80% beating cardiomyocytes were observed and the beating frequency of cells was 50 beats/min after 3 days. The purity of cardiomyocytes was 75 %. Conclusion: This method of isolation and culture of cardiomyocytes in neonatal rat was mature.
出处 《心血管康复医学杂志》 CAS 2006年第6期538-540,共3页 Chinese Journal of Cardiovascular Rehabilitation Medicine
基金 湖北省教育厅资助项目(2004Q001)
关键词 心肌 大鼠 细胞培养 Myocardium Rat Cell, cultural
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参考文献4

  • 1Bes S,Roussel P,Laubriet A,et al.Influence of deep hypothermia on the tolerance of the isolated cardiomyocyte to ischemia-reperfusion[J].J Mol Cell Cardiol,2001,33(11):1973-1988.
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