摘要
目的探讨丹参对大鼠移植胰腺缺血再灌注损伤的保护作用及机制。方法建立SD大鼠同种异位全胰十二指肠移植模型，供体胰腺分别用肝素平衡盐液或肝素平衡盐液添加丹参进行低温（0℃～4℃）灌注保存60min。光镜、电镜观察术后再灌注12h胰腺腺泡细胞结构改变，测定血清中脂肪酶、淀粉酶及胰腺组织的细胞凋亡指数，应用免疫组化染色检测Bcl-2，Bax蛋白表达。结果丹参组胰腺组织结构损伤明显轻于对照组，血清中脂肪酶、淀粉酶明显低于对照组；对照组凋亡指数明显大于正常组（P〈0．01），丹参组凋亡指数明显小于对照组（P〈0．01）：丹参组Bcl-2蛋白表达明显高于对照组（P〈0．01），Bax蛋白表达明显低于对照组（P〈0．01）。结论丹参可能通过上调Bcl-2蛋白表达，下调Bax蛋白表达，对大鼠移植胰腺缺血再灌注损伤起保护作用。
Objective To investigate the protective effects of salvia miltiorrhiza （SM） on rat＇s ischemia-reperfusion injury after pancreaticoduodenal transplantation. Methods The allogeneic SD rats model of heterotopic total pancreaticoduodenal transplantation was estabhshed. The isolated pancteases of rats were peffused and stored with calparine balanced solution in which SM were added as an additive agent in the SM group. No SM were added to calparine balanced solution in the control group. The organs were stored at0 ℃ -4 ℃ for 60 min, plasma concentrations of amylase and lipase were examined and histological observation were performed at 12 h after reperfusion. Apoptofic cells were stained by TUNEL technique. Expression of Bax and Bcl-2 protein in pancreatic tissue were detected with immunohistochemical method. Results Histomorphological changes of pancreas in SM group were much lighter than those in control group. The plasma concentralions of amylase and hpase were significantly lower in SM group than those in control group （ P 〈 0.01 ）. The expressions of Bcl-2 protein in SM group were significantly higher than those in control group （ P 〈 0.01 ）. The expressions of Bax proteein in SM group were significantly lower than those in control group （ P 〈 0.01 ）. Conclusion SM can decrease pancreatic ischemia-reperfusion injuries by up-regulating Bcl-2 and down-regulating Bax.
出处
《肝胆胰外科杂志》
CAS
2006年第6期347-349,共3页
Journal of Hepatopancreatobiliary Surgery
关键词
胰十二指肠移植
凋亡
丹参
BCL-2
BAX
pancmaficoduodenal transplantation
apoptosis
salvia miltiorthiza
Bcl-2
Bax
