摘要
研究了斯达油脂酵母Ｕ９０１８产胞外多糖的适宜条件：培养基组成（ｇ／Ｌ）：蔗糖１００，蛋白胨７，ＫＨ２ＰＯ４３５，Ｋ２ＨＰＯ４·３Ｈ２Ｏ３．５，ＭｇＳＯ４·７Ｈ２Ｏ０．２，ＭｎＳＯ４·Ｈ２Ｏ０．０１，ＦｅＳＯ４·７Ｈ２Ｏ０．０１，ＮａＣｌ０．０１，初始ＰＨ６．５。装液量为５００ｍｌ三角瓶装培养基７０ｍｌ，接种量５％。在上述条件下，２５℃振荡（２００ｒ／ｍｉｎ）培养１０８ｈ，纯多糖产量可达２３．２ｇ／Ｌ。
Suitable conditions have been studied for exopolysaccharide production by Lipomycesstarkeyi U9018.Composition of the suitable medium was as following (g/ L):Sucrose 100,Peptone 7, KH_2PO_4 3.5.K_2HPO·3H_2O 3.5,MgSO_4·7H_2O 0.2,MnSO_4·H_2O 0.01,FeSO_47H_2O 0.01,NaCl 0.01.The optimal initial PH for exopoly saccharide production was PH 6.5.Theoptimal medium volume in 500 ml flask was 70 ml.Under these conditions,the yield ofexopolysaccharide was as high as 23.2 g/L on rotary shaker at 25℃ for 108 h.
出处
《微生物学通报》
CAS
CSCD
北大核心
1996年第5期268-271,共4页
Microbiology China
