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凝胶板植酸酶活力检测 被引量:1

DETECTION OF PHYTASE IN GELS
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摘要 将1mm厚凝固于复印膜上的水琼脂(15%~2.0%)凝胶板侵入含12mmol/L植酸钠的Gly—HCl缓冲液中达1h以上,取出干至胶表面无水迹,于上加Aspergillussp.59—2植酸酶或与电泳后的凝胶板紧贴10~60min。然后水平置于恒温水浴锅中反应一定时间,取出浸入1mol/LH2SO4-2%(NH4)6Mn7O24-10%FeSO4溶液显色2~5min。该法简便、快速、灵敏,反应15min即可检出每cm2低于0.0045μ的植酸酶。此法适用于微生物的初筛、酶谱分析及酶比活的比较。 A sheet of agar gel(1.5%~2.0%,1mm thickness)on copy film was soaked in Gly-HCl buffer containing 1 .2m mol/L sodium phytate for over 1h and then taken out.After the moisture on thegel surface disappeared, phytase was spotted on or an electrophoresed polyacryamide gel contaillingphytase was placed in contact with it for 10~60min,then the agar gel was horizontally incubated inwater bath for 15 min at 37oC,finally the gel was immersed in lmol/L H2SO4-2%(NH4)6Mo7O24-10% FeSO4 for 2~5min.to develop its colour.This assay is simple, rapid andhighsensitive, as little as 0.0045 μ phytase on one cm2 of gel could be detected,which is suitable forscreening microorganism,zymogram analysis and comparing specific activities of phytases.
作者 江均平
出处 《微生物学通报》 CAS CSCD 北大核心 1996年第5期316-317,共2页 Microbiology China
基金 国家自然科学基金
关键词 植酸酶 酶谱 活力检测 Phytase,Zymogram,Detection ofphytase in agar gel
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  • 1江均平,微生物学报,1996年,36卷,6期,432页

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