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APA微囊包裹的牙髓干细胞成牙本质作用的研究 被引量：3

Study of the capability of APA-microcapsulated dental pulp stem cells on generating dentin
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摘要 目的:观察海藻酸钠-多聚赖氨酸-海藻酸钠(APA)微囊包裹的牙髓干细胞移植在Scid小鼠腹腔内后成牙本质的作用,探讨APA作为牙齿组织工程支架材料的可行性。方法:用APA微囊包裹第二代人牙髓干细胞,植入Scid鼠腹腔内,植入6周后取材,通过检测被APA微囊包裹的牙髓干细胞是否表达牙本质基质特异性蛋白,观察牙本质形成情况。结果:APA微囊包裹的牙髓干细胞在植入6周后可以检测到特异性牙本质基质蛋白-牙本质涎磷蛋白的形成。结论:用APA作为支架材料,采用组织工程方法包裹的牙髓干细胞可以形成牙本质基质,牙髓干细胞和APA用于组织工程牙本质的构建具有广阔前景。 Objective： To observe the character of alginate-polysine-alginate （APA）-microcapsulated dental pulp stem cells （DPSCs） and its capability on generating dentin. Methodes： The APA microcapsulated humen DPSCs were transplanted into the mice abdominal cavity. APA microcapsules were recovered after six weeks of intranperitoneal injection.RT-PCR methods were used to observe the expression of DSPP and examine the generation of dentin. Results： DSPP express were detected after six weeks of implantation of, APA microcapsulated DPSCs. Conclusion： The polysine-alginate APA-microcapsulated dental pulp stem ceils （DPSCs） have the capability to generate dentin through tissue engineering methods.
作者 王瑛慧 王万永 贾智
机构地区 天津医科大学口腔医院牙体牙髓科 天津市第一中心医院口腔科
出处 《天津医科大学学报》 2006年第4期548-550,共3页 Journal of Tianjin Medical University
关键词 APA微囊 牙髓干细胞 组织工程 APA microcapsules Dental pulp stem cells Tissue engineering
分类号 R781.05 [医药卫生—口腔医学] R318.08 [医药卫生—生物医学工程]
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参考文献12

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共引文献7

同被引文献53

引证文献3

二级引证文献20

天津医科大学学报
2006年 第4期

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