摘要
采用随机扩增多态性DNA（RAPD）技术检测镉（cd）胁迫对蚕豆幼苗根尖DNA多态性的影响。结果表明，不同浓度（2．5、5和15mg·L^-1）镉处理7d后，蚕豆幼苗根伸长及根系中可溶性蛋白质含量均受到了抑制。选用12条寡核苷酸引物（10bp）对蚕豆幼苗根尖细胞中基因组DNA进行RAPD扩增，其中有6个引物产生特异性PCR产物。对照组蚕豆幼苗根尖基因组DNA的RAPD图谱中可分辨出86条RAPD谱带，其分子量为200—2520bp。处理组与对照组RAPD图谱之间存在明显差异，且与镉浓度之间存在剂量-效应关系。这些结果表明，镉影响蚕豆幼苗根尖细胞中基因组模板的稳定性，故利用RAPD技术获得的DNA多态性变化可作为检测镉黄传毒性效应的生物标记物。
By using random amplified polymorphic DNA （RAPD） technique, this paper studied the effects of Cd stress on DNA polymorphism in root tip of Vicia faba seedlings. The results showed that after 7 days of 2. 5 - 15 mg Cd·L^-1 stress, the root elongation growth was inhibited, and the total soluble protein content in root tip was decreased. A total of six oligonucleotide primers （10 bp） were found to produce unique polymorphic band patterns, and subsequently used to produce 86 bands of 200 -2 520 bp in molecular size in the normal root tip of V. faba seedlings. The RAPD profiles showed obvious differences between normal and exposed plantlets, and the change effect was dose-dependent. The genomic template stability was significantly affected by Cd pollution, and the DNA polymorphisms detected by RAPD technique could be used as a biomarker for detecting the genotoxic effects of Cd stress on plants.
出处
《生态学杂志》
CAS
CSCD
北大核心
2007年第1期35-39,共5页
Chinese Journal of Ecology
基金
国家自然科学基金项目(20377043
20337010)
国家重点基础研究发展规划资助项目(2004CB418506)
