摘要
以活性干酵母为菌种,采用摇瓶培养,通过单因素试验和正交试验,分析了发酵培养基各成分对酵母生长和富铬性能的影响,确定了发酵培养基最优配方,即酵母浸出粉10g/L、蛋白胨10g/L、蔗糖90g/L、KH2PO42.0g/L、MgSO41.5g/L和微量元素1mL/L。在该营养条件下发酵生产富铬酵母,生物量可达10.308g/L,有机铬含量达到5190.68μg/g,有机铬总含量为53504.7μg/L,富铬效果较好且稳定。
By using single factor tests and the orthogonal design, biomass and Cr content of yeast influenced by the factors of fermentation medium was studied. The optimum fermentation medium was determined as： yeast extract powder 10 g/L, peptone 10 g/L, sucrose 90 g/L, KH2PO4 2.0 g/L, MgSO4 1.5 g/L and trace element 1ml/L. Under the initial optimized fermentation medium, biomass, organic Cr content and the total organic Cr content of chromiumenriched yeast reached 10.308 g/L, 5 190.68 μg/g, and 53 504.7 μg/L respectively, the concentrating capability was distinct and steady.
出处
《食品研究与开发》
CAS
北大核心
2007年第1期67-71,共5页
Food Research and Development
关键词
活性干酵母
铬
营养条件
active dry yeast
chromium
nutrition condition
