摘要
目的:观察大鼠脊髓损伤后神经细胞黏附分子的表达,分析其与神经修复的关系。方法:实验于2006-02/05在兰州大学第二附属医院骨科研究所完成。成年雄性Wistar大鼠36只,分为空白对照组(n=3)、假手术组(n=3)和损伤组(n=30)。损伤组采用Allen法制成急性外伤模型。分别于术后1,3,5,7,10d麻醉下处死。假手术组仅实施手术不行打击,空白对照组不做任何处理,均在实验初处死。对各组大鼠在处死前以改良的Tarlov评分检测行为及肢体运动功能,观察伤后各时相点大鼠后肢的活动、肌力等,以此评定损伤后大鼠运动功能的恢复情况。测量各组各时间点脊髓灰质中阳性反应细胞并测其灰度值,用免疫组织化学的方法测定脊髓片神经细胞黏附分子的表达。结果:纳入动物数量为36只,损伤组死亡4只,最终进入结果分析32只。①损伤组脊髓损伤后1d改良的Tarlov评分低于空白对照组和假手术组(P<0.01),损伤后10d高于损伤后1d(P<0.01),但评分仍低于假手术组(P<0.01)。②损伤后1d肉眼可见脊髓组织肿胀明显,软脊膜紧绷。光镜下可见中心灰质血管破裂。白质神经纤维水肿,轴突与髓鞘之间间隙增大,1d后肿胀逐渐加重。损伤5d后脊髓肿胀逐渐消退,10d后轴突及髓鞘已退变成空泡。③空白对照组、假手术组脊髓灰质神经细胞黏附分子的表达甚少,损伤组1,3,5,7,10d表达增加,与空白对照组、假手术组比较,差异有显著性意义[(175.38±4.51),(163.15±5.98),(196.28±6.57),(217.42±2.36),(228.36±2.62),(248.31±3.47)(245.28±3.87),P<0.01]。结论:脊髓损伤后神经细胞黏附分子表达增加,提示神经细胞黏附分子可能参与损伤后神经的修复。
AIM： To investigate the expression of neural cell adhesion molecule （NCAM） in rats with spinal cord injury and analyze its relationship with the neural repairing.
METHODS： The experiment was conducted in the Department of Orthopedics, Second Hospital Affiliated to Lanzhou University between February and May 2006. Thirty-six male adult Wistar rats were randomly divided into the blank control group （n =3）, sham-operation group （n =3） and the injury group （n =30）. Rats in the injury group were established into acute injury models with Allen method and then executed under anesthesia respectively at 1, 3, 5, 7 and 10 days after the operation. Rats in the sham-operation group only received operation without knocking, while rats in the blank control group received no treatment, and rats were executed at the beginning of experiment. The behavior and the motor ability of limbs were evaluated by modified Tarlov method in all groups, and the motion and muscular strength of the posterior limbs in rats were observed in all time-points after the injury so as to evaluate the recovery of rat＇s motion. The positive cells in gray nucleus and the gray scale in each time-point were measured in all groups, and immunohistochemistry was used to study the expression of the NCAM.
RESULTS： Of 36 enrolled animals, 4 rats died in the injury group, and there were 32 rats entered the final analysis. ① The Tarlov score at one day after the spinal cord injury was lower in the injury group than that in the blank control group and sham-operation group （P 〈 0.01）, and it was higher at 10 days after the injury than that at 1 day after the injury （P〈 0.01）, while the score was still lower than that in the sham-operation group （P〈 0.01）. ② At one day after the injury, swelling could be obviously seen in myeloid tissue by nude eyes and the spinal pia mater was in tension. Under the light microscope, there was angiorrhexis in the central gray matter, and edema was found in the nerve fiber of white matter with increased gaps between the axis and myelin sheath, moreover, the engorgement was aggregated day later. Five days after the injury, the spinal cord engorgement gradually disappeared, and the axis as well as the myelin sheath turned into vacuoles 10 days later. ③ The expressions of NCAM in the gray substance of spinal cord were less in the blank control group and sham-operation group, while those in the 1, 3, 5, 7 and 10 days injury groups were increased, and there were significant differences in comparison with the blank control group and the sham-operation group [（175.38±4.51 ）, （163.15±5.98）, （196.28±6.57）, （217.42±2.36）, （228.36±2.62）, （248.31±3.47）（245.28±3.87）,P 〈 0.01].
CONCLUSION： The expression of NCAM increases after spinal cord injury, which indicates that NCAM may participate in the neural repair after the injury.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第2期297-300,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
