摘要
为了探讨用激光防治血管成形术后再狭窄的可能性,以510nm激光照射体外培养的血管平滑肌细胞,以DNA片断末端标记法观察细胞凋亡现象。结果显示,50和75J/cm2照射后4小时SMC出现凋亡现象,24小时凋亡最为显著,凋亡率分别为9.4%和6.1%。表明激光直接照射能引起平滑肌细胞凋亡。
We investigated the effect of laser irradiation to induce apoptosis of vascular smooth muscle cells(SMC). Rabbit SMC, isolated from the thoracic arota, were cultured in DMEM containing 10% fetal calf serum. SMC from passages 5 and 6 were irradiated by 510 nm copper vapor laser with the power densities of 25, 50, 75, 100, 200 mW/cm 2 and the energy densities of 25, 50, 75, 100, 200 J/cm 2. Apoptosis of SMC were identified by specific labeling of nuclear DNA fragment (TUNEL) at 4, 12, 24, 48 and 72 hours after irradiation and the necrotic cells were determined by the trypan blue dye exclusion test. It was found that SMC began to undergo apoptosis at 4 hours after irradiation by 50 and 75 J/cm 2 and the number of apoptotic cells reached a maximum at 24 hours. This result indicated that 510 nm copper vapor laser irradiation may induce apoptosis of SMC and it should attract attention as a possible approach to reduce coronary restenosis.
出处
《中国激光医学杂志》
CAS
CSCD
1996年第3期136-139,共4页
Chinese Journal of Laser Medicine & Surgery
关键词
激光
血管平滑肌细胞
细胞凋亡
Laser, Vascular smooth muscle cells, Apoptosis