摘要
目的机械振荡法制备并评价适用于超声空化栓塞肿瘤微循环的负电荷脂膜超声造影剂。方法采用机械振荡法在不改变脂质及辅料总量前提下,通过改变配方中脂质成分比例以及聚乙二醇(PEG)的用量,制备三种配方超声造影剂,分别观察并检测超声造影剂的浓度、表面电位、pH值、粒径及分布。结果三种配方对造影剂的浓度、稳定性几乎无影响,但对其的电荷特性有明显影响。三种配方所制备微泡的粒径均值分别为1066.6nm、1605.4nm、1552.5nm;微泡表面电位均值:在蒸馏水中分别为-33.8mV、-57.6mV、-66.7mV,在生理盐水中分别为0.1mV、-37.5mV、-37.8mV。与配方1比较,配方2、3所制备微泡大小适中、表面带有更多负电荷(P<0.01),配方2、3间比较无明显差异(P>0.05)。结论采用机械振荡法,增加脂质成分中负电荷磷脂的比例,可以制备出表面带有更多负电荷的超声造影剂,为超声空化栓塞肿瘤微循环提供了实验基础。
Objective To increase the negative surface charge of microbubbles by using more negative charged lipid in the lipid formula and assess its physiochemical properties. Methods Three different proportions of lipid components were designed to test the preparation of microbubbles with different surface charge. The first formula used more neutral lipid than negative charged lipid with polyethylene glycol （PEG）. The second formula used more negative charged lipid than neutral lipid with PEG. The third plan used more negative charged lipid with the same amount of mannitol to replace PEG. Three kinds of microbubbles were prepared by a high-speed mechanical vibrator. Then, the microbubbles were sent to microscopy and Zeta potential for the shape, size, concentration, distribution and surface charge. Results There was no difference between the three microbubbles each other in the bubble concentration and in the stability in vitro,while the difference between their electronic properties was obvious. The electronic potentials of the two anionic lipid microbubbles were - 37.5 mV, -37.8 mV in saline and -57.6 mV, -66.7 mV in distilled water. The bubbles average diameters were 1605.4 nm, 1552. 5 nm respectively. In control, another lipid microbubble with a neutral surface charge had only 0.1 mV of electronic potential in saline and -33.8 mV in distilled water, and the average diameter was 1066.6 nm. Conclusion Anionic lipid microbubbles with a net negtive charge could be prepared by changing the proportion of lipids. This design of the new anionic microbubble was specific for therapeutic purpose.
出处
《中国医学影像技术》
CSCD
北大核心
2006年第12期1773-1776,共4页
Chinese Journal of Medical Imaging Technology
基金
国家自然科学基金资助项目(30471654)。
关键词
超声造影剂
机械振荡
负电荷
Ultrasound contrast agent
Mechanical vibration
Negative charge
