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乙肝病毒X基因对肝癌细胞表达RhoC的影响 被引量：4

The effect of HBV X gene vector on the expression of RhoC protein in HEPG2 cell line
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摘要 目的探讨X基因对肝癌细胞表达RhoC基因的影响。方法用定向克隆的方法构建X基因的真核表达载体pcDNA 3.1-X,脂质体转染HEPG 2细胞;潮霉素选择培养稳定表达X基因的HEPG 2-X细胞;免疫组化鉴定HEPG 2-X细胞RhoC蛋白表达。结果构建的真核表达载体pcDNA 3.1-X在HEPG 2细胞中有稳定表达,HEPG 2-X细胞表达RhoC蛋白增强。结论体外条件下,X基因可促进肝癌HEPG 2细胞RhoC表达增强。这可能与肝癌的侵袭、转移有关。 Objective To explore the effect of HBV X gene in the expression of RhoC gene in HEPG2 cell line. Methods The whole-length X gene was directly cloned to pcDNA3. 1 vector. The recombinant vector （ pcDNA3. 1 -X ） was transfected into HEPG2 cells after selection with hygromycin, steady clones （ HEPG2 -X cells ） were obtained. The expression of RhoC protein was analysed with immunohistochemical stain. Results The recombinant plasmid could steadily express X protein efficiently, and the expression of RhoC protein in HEPG2-X cell was enhanced. Conclusions HBV X gene can enhance the expression of RhoC protein in vitro. It may be related to invasiveness and metastasis of hepatocellular carcinoma.
作者 郑岩松 吕新生 曾永毅 林永堃 林旭
机构地区 福建医科大学附属第一医院普通外科 中南大学湘雅医院普通外科 福建医科大学分子医学中心
出处 《中国普通外科杂志》 CAS CSCD 2006年第12期923-926,共4页 China Journal of General Surgery
基金 福建省自然科学基金(c0310013) 福建省卫生厅青年科研基金(2002-01-04)资助
关键词 基因 X 基因 Rhoc 肝肿瘤 实验性 基因表达 Genes, X Genes, Rhoc Liver Neoplasms, Experimental Gene Expression
分类号 R73-3 [医药卫生—肿瘤] R735.7 [医药卫生—肿瘤]
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参考文献9

  • 1Cougot D,Neuveut C,Buendia MA.HBV induced carcinogenesis[J].J Clin Virol,2005,34(1):S75 -78.
  • 2Ou DP,Tao YM,Chang ZG,et al.Hepatocellular carcinoma cells containing hepatitis B virus X protein have enhanced invasive potential conditionally[J].Dig Liver Dis,2006,38 (4):262-267.
  • 3Zhang X,Zhang H,Ye L.Effects of hepatitis B virus X protein on the development of liver cancer[J].J Lab Clin Med,2006,147(2):58 -66.
  • 4Clark E,Golub T,Lander E,et al.Genomic analysis of metastasis reveals an essential role for RhoC[J].Nature,2000,406(6795):532 -535.
  • 5Kleer CG,van Golen KL,Zhang Y,et al.Characterization of RhoC expression in benign and malignant breast disease:A potential new marker for small breast carcinomas with metastatic ability[J].Am J Pathol,2002,160(2):579 -584.
  • 6石铮,郑岩松,曾金华,刘景丰,林旭.RhoC基因及蛋白在胃癌肝转移中的表达及意义[J].中华实验外科杂志,2002,19(4):310-311. 被引量：23
  • 7郑岩松,吕新生,林永堃,石铮.RhoC基因在原发性肝癌中的表达及其意义[J].中国普通外科杂志,2002,11(9):528-530. 被引量：7
  • 8Pille JY,Denoyelle C,Varet J,et al.Anti-RhoA and anti-RhoC siRNAs inhibit the proliferation and invasiveness of MDA-MB-231breast cancer cells in vitro and in vivo[J].Mol Ther,2005,11(2):267 -274.
  • 9Han J,Yoo HY,Choi BH,et al.Selective transcriptional regulations in the human liver cell by hepatitis B viral X protein[J].Biochem Biophys Res Commun,2000,272 (2):525-530.

二级参考文献11

  • 1[1]Madaule P, Axel R. A novel ras-related gene family[J]. Cell,1985,41(1):31-40.
  • 2[2]Chardin P,Madaule P,Tavitian A,et al. Coding sequence of human Rho cDNAs clone 6 and clone 9[J]. Nucleic Acid Res, 1988,16(): 2717.
  • 3[3]Clark E, Golub T, Lander,E, et al. Genomic analysis of metastasis reveals an essential role for RhoC[J]. Nature, 2000,406(): 532-535.
  • 4[4]Kleer CG, van Golen KL, Zhang Y,et al.Characterization of RhoC expression in benign and malignant breast disease : A potential new marker for small breast carcinomas with metastatic ability[J]. Am J Pathol, 2002, 160(2):579-584.
  • 5[5]Takamura M, Sakamoto M, Genda T, et al. Inhibition of intrahepatic metastasis of human hepatocellular carcinoma by Rho-associated protein kinase inhibitor Y-27 632[J]. Hepatology, 2001,33(3):577-581.
  • 6[6]Genda T,Sakamoto M,Ichida T,et al.Cell motility mediated by Rho and Rho-associated protein kinase plays a critical role in intrahepatic metastasis in human hepatocellular carcinoma[J]. Hepatology, 1999,30():1027-1036.
  • 7[7]Hiroshi O,Ssiji S,Tatsushi K,et al.Genome-wide analysis of gene expression in human hepatocellular carcinomas using cDNA microssary:Identification of genes involved in viral carcinogenesis and tumor progression[J].Cancer Res,2001, 61():2129-2137.
  • 8Kleer CG,Van Golen KL,Zhang Y,et al.Characterization of RhoC expression in benign and malignant breast disease: a potential new marker for small breast carcinomas with metastatic ability[].American Journal of Pathology.2002
  • 9Clark EA,Golub TR,Lander ES,et al.Genomic analysis of metastasis reveals an essential role for RhoC[].Nature.2000
  • 10Takamura M,Sakamoto M,Genda T,et al.Inhibition of intrahepatic metastasis of human hepatocellular carcinoma by Rho-associated protein kinase inhibitor Y-27632[].Hepatology.2001

共引文献27

同被引文献23

引证文献4

二级引证文献13

中国普通外科杂志
2006年 第12期

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