摘要
借助cDNA-AFLP技术分离到15个与白菜离体春化反应相关的表达序列标签(EST)。Blast结果表明:低温春化处理抑制性表达的12个EST,多与异化作用相关基因同源性较高;春化诱导表达的3个EST,分别与编码核糖体蛋白的亚基(或与自主开花途径相关的FAC基因连锁的因子),ATPase的亚基,小G-蛋白亚基的基因同源性较高。
Fifteen ESTs associated with vernalization in vitro of Chinese cabbage-pak-choi （Brassica campestris L. ssp. chinensis Makino） were isolated by cDNA-AFLP. Blast results showed that 12 ESTs downregulated by vernalization had high similarity to genes encoding enzymes involved in the disassimilation. 3 ESTs upregulated had high similarity to genes with positive effect on vernalization, including ribosomal protein subunit related to the synthesis of priotein or the linkage factor of FAC involved in auto flowering pathway, the subunit of ATPase, and the subuint of small-G-protein.
出处
《园艺学报》
CAS
CSCD
北大核心
2006年第6期1341-1344,共4页
Acta Horticulturae Sinica
基金
浙江省重大科技攻关项目(2005C12019-02)
国家自然科学基金项目(30370975)
关键词
白菜
春化
离体
基因
CDNA-AFLP
Chinese cabbage-pak-choi
Brassica campestris ssp. chinensis
Vernalization
In vitro
Gene
cDNA-AFLP
