摘要
目的:通过对尿儿茶酚胺代谢产物去甲肾上腺素、甲氧基肾上腺素的测定,综合分析其对嗜铬细胞瘤的早期临床诊断价值。方法:利用酶联免疫分析法和传统柱层析法,对正常人和临床诊断为嗜铬细胞瘤及肾上腺占位病变并伴有阵发性高血压患者的24h尿去甲基肾上腺素/甲氧基肾上腺素和3-甲氧基-4-羟基苦杏仁酸(VMA)进行测定。结果:与VMA相比,24h尿去甲基肾上腺素/甲氧基肾上腺素在嗜铬细胞瘤患者中的测定值要显著高于其他肾上腺占位性病变伴高血压患者和正常人群,二者方法学有显著性差异。结论:酶联免疫分析法检测24h尿去甲基肾上腺素/甲氧基肾上腺素具有灵敏度高、特异性好的特点,为临床从肾上腺占位性病变并伴有阵发性高血压患者中筛查嗜铬细胞瘤提供了一种有价值的参考方法。
Objective： To analyze the value for early diagnosing pheochromocytoma by measuring the catecholamine＇s metabolites normetanephrine/metanephrine（NMN/MN） in urine. Methods： NMN/MN and 3-methoxy-4-hydroxylmandelic acid （VMA） were determined to healthy subjects and hypertension accompanying adrenal gland that holds pathological changes in 24 h urine by the ELISA and the column chromatography. Results： The measurement of NMN/MN in 24 h urine in pheochromocytoma was in evidence higher than VMA in hypertension accompanying adrenal gland that holds pathological changes and healthy subjects, and was markedly difference to VMA. Conclusion： The measurement of NMN/ MN by ELISA in 24 h urine is a sensitive and special way to mensurate pheochromocytoma and can filtrate pheochromocytoma from hypertension accompanying adrenal gland that holds pathological changes.
出处
《生物技术通讯》
CAS
2006年第6期918-919,共2页
Letters in Biotechnology
