摘要
目的探讨乳腺癌组织中缺氧诱导因子-1α(hypox ia-induc ib le factor 1 a lpha,H IF-1α)的表达及其与增殖细胞核抗原(pro liferating ce ll nuclear an tigen,PCNA)、血管内皮生长因子(vascu lar endothe lia l grow th fac-tor,VEGF)及临床病理因素的关系。方法采用免疫组化SP法检测乳腺纤维腺瘤、普通乳腺增生组织和乳腺癌中H IF-1α、PCNA、VEGF蛋白的表达。结果H IF-1α蛋白在乳腺纤维腺瘤、普通乳腺增生组织中不表达;乳腺导管内原位癌中阳性率55%(11/20),浸润性乳腺癌中85%(51/60);乳腺癌中VEGF阳性率81.3%(65/80);乳腺癌中PCNA阳性率75%(60/80),其中原位癌中65%(13/20),浸润癌中78.3%(47/60)。乳腺癌中H IF-1α表达与VEGF(r=0.762,P<0.01)、PCNA(r=0.693,P<0.01)均呈正相关;H IF-1α在淋巴结转移与否、不同雌激素受体状态及组织学分级中表达的差异有统计学意义。结论H IF-1α及其靶基因VEGF在乳腺癌组织中明显上调,H IF-1α表达与肿瘤细胞增殖、淋巴结转移、雌激素受体状态及组织学分级相关,提示H IF-1α对乳腺癌的发生发展起重要作用,有望成为肿瘤放、化疗及生物治疗的一个新靶点。
Objective To investigate the expression of hypoxia- inducible 1 alpha （HIF-1α in human breast cancer and its relationship with vascular endothelial growth factor （VEGF） protein, proliferating cell nuclear antigen （ PCNA ） protein, other tumor biomarkers and clinical pathologic factors. Methods The immunohistochemical staining （SP） was used to measure the expression of HIF-1α,VEGF and PCNA in human breast fibroadenoma, usual hyperplasia and breast carcinoma. Results HIF-1α was not found expressing in breast fibroadenoma and hyperplastic lesions. In contrast, the positive rate of HIF-1α was found in the ductal carcinoma in situ 55% （DCIS, 11/20） and the invasive breast carcinoma 85% （51/60）. VEGF positivity in breast carcinoma was 81.3% （65/80）. The total positive rate of PCNA in breast carcinoma was 75% （60/80）, that in DCIS was 65% （13/20） and that in invasive carcinoma was 78. 3% （47/60）. Increased levels of HIF-1αwere statistically significantly associated with increased expression of VEGF （r=0. 762, P〈0. 01） and PCNA （r = 0. 693, P〈 0. 01）. Conclusion The upregulated expression of HIF-1α and VEGF in breast carcinoma has a close relationship with tumor angiogenesis, tumor cell proliferation, lymph node metastasis, ER status and stages of histology. This suggests that HIF-1α plays an important role in the carcinogenesis and progression of breast carcinoma ; is wished to become a new target for radiotherapy, chemotherapy and biotherapy of tumor, which will offer the new ways to diagnosis and treatment of tumor.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2007年第1期60-63,共4页
Journal of Sichuan University(Medical Sciences)
关键词
乳腺癌
缺氧诱导因子-1Α
肿瘤细胞增殖
血管形成
Breast carcinoma Hypoxia-inducible factor 1 alpha （HIF-1α） Tumor cell proliferation Angiogenesis
