摘要
通过响应曲面法(responsesurfacemethodology,RSM)研究了温度、磁场强度及曝磁时间这三个磁场处理的不同因素,对于由酿酒酵母中分离出的超氧化物歧化酶(EC1.15.1.1)活性效果的影响。这种多变量统计方法可用于进行酶分析,并可研究体系内不同变量的交互作用,建立了可用于预测实验数据的二次多项式模型。失拟项不显著,决定系数(R2)大于94%。根据模型优化出可以取得最大酶活性的工艺参数为:温度15.5℃,磁场强度0.95T,曝磁时间64.5min。
The effect of three variables （temperature, magnetic intensity, and exposure time） on the activity of superoxide dismutase （EC 1.15.1.1） isolated from Saccharomyces cerevisiae was studied by Response Surface Methodology （RSM）. This multivariate methodology offers an empirical approach to the study of enzyme assays and allows to detect the interaction between different variables of the system. Analysis of variance （ANOVA） showed that a second order polynomial model （SOP） generated response surface and contour plots, and the model may predict the experimental data. Lack-of-fit tests did not result in a significant F-value. Determination coefficients （RS） were greater than 94%. The optimum treating parameters were obtained as： temperature, 15.5℃; magnetic intensity, 0.95T; time of magnetic field exposure, 64.5 minutes.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2006年第12期62-66,共5页
Food Science
基金
国家自然科学基金重点项目(20436020)
国家自然科学基金青年基金项目(20306007)
广东省自然科学基金重点项目(04105934)
关键词
超氧化物歧化酶
酿酒酵母
响应曲面法
superoxide dismutase
Saccharomyces cerevisiae
response surface methodology
