摘要
建立了一种凝胶渗透色谱-高效液相色谱同时检测辣椒及其制品中对位红和五种苏丹红的方法。样品经环己烷-乙酸乙酯(体积比为1:1)提取,凝胶渗透色谱(GPC)净化,采用Diamond柱(250mm×4.6mmi.d.,5μm)分离,以乙腈:水(体积比为95:5)为流动相,流速1.0ml/min;用紫外检测器检测,检测波长500nm。对位红和苏丹红0.01～10.0mg/L时线性关系良好,加标回收率为90%～102%,相对标准偏差为1.6%～5.2%,六种染料的检出限均为0.01mg/kg。
A GPC-HPLC method for the determination of Para Red and Sudan Red Dyes in capsicum and capsicum products was described. Para Red and Sudan Red Dyes was extracted from samples with cyclohexane-ethyl acetate （1：1, VN）. The extract was cleaned up with gel permeation Chromatography. The analysis was performed on a Diamond column （250mm ～4.6mm i.d.5μm） with acetordtrile-water （95：5, V/V） as the mobile phase at a flow rate of 1.0ml/min, detection at 500 nm by UV. The calibration curves present good linear relationship in the range of 0.01 ～10og/ml, and the correlation coefficients were above 0.9998 for all compounds. Recoveries of all dyes added were 90%～102%respectively. Detection limit of the method for Para Red and Sudan Red Dyes was 0.01mg/kg.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2006年第12期611-613,共3页
Food Science
关键词
凝胶渗透色谱
高效液相色谱
辣椒
苏丹红
对位红
gel permeation chromatography（GPC）
high performance liquid chromatography（HPLC）
capsium
Para
