摘要
采用地高辛标记探针对高分化和低分化鼻咽癌细胞中由EB病毒编码的RNAs(Epstein-Barrvirus encoded RNA,EBERs)进行了检测。结果显示,在高分化鼻咽癌细胞株CNE-1和低分化鼻咽癌细胞株CNE-2,CNE-3细胞中均有EBERs,EBERs位于细胞核中,且含量丰富,用原位杂交技术检测EBERs可望作为鼻咽癌早期诊断工具。
Human nasopharyngeal carcinoma cell lines (CNE-1, CNE-2, CNE-3) were detected by in situ hybridization with Dig-labelled EBERs probe. Results showed that in both well and poorly differentiated NPC there had EBV DNA presentation, which were richly located, specifically in the nuclei of the cancer cells. Positive rate were>92%. The EBERs were detected as earily as 36h after EBV infection and it is comparatively stable. In situ hybridization technique is likely to be an effective method in the early dignosis of NPC.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
1996年第4期349-351,共3页
Chinese Journal of Experimental and Clinical Virology
基金
国家自然科学基金资助
关键词
原位杂交
鼻咽肿瘤
RNA
病毒
EB病毒
In situ hybridization Epstein-Barr virus Nasopharyngeal neoplasms RNA, viral
