摘要
本文测定了猪肺炎霉形体膜上ATP酶的活性,同时就温度、pH及一些金属离子对该ATP酶活性的影响进行了研究。结果表明:猪肺炎霉形体膜上ATP酶比活力为9.232左右。在25~40℃温度范围内酶活性较稳定。pH适应范围为6.5~8之间,以pH7.6为最适。Mg2+、Mn2+、Zn2+对酶均有一定程度的激活作用,而Ca2+、Na+-K+具有不同程度的抑制作用。
The membrane-bound atpase activity of mycoplasma hyopneumoniae were tested and the effect of temperature,PH and some metal-ions on the ATPase activity were studied.The results show:the ATPase specific activity on mycoplasma hyopneumoniae is about 9. 232,Enzyme activity is stable comparativily on the condition of 25 C ̄40 C.PH range from 6.5 to 8,and the fittest value is 7.6.Mg2+,Mn2+and Zn2+all have certain stimulus function.Whereras Ca2+,Na+-K+have the different extent inhibitory effect.